Yamamoto S, Kubota Y, Tsuji K, Yanagitani K, Takaoka M, Kin H, Ogura M, Inoue K
Third Department of Internal Medicine, Kansai Medical University, Osaka, Japan.
J Gastroenterol Hepatol. 1998 Apr;13(4):405-11. doi: 10.1111/j.1440-1746.1998.tb00655.x.
The effect of obstructive jaundice on local neutrophil accumulation in response to inflammatory stimulus was investigated in rats. Obstructive jaundice was produced by bile duct ligation for 7 days. Zymosan (200 mg) was injected intraperitoneally and 4h later myeloperoxidase activity in the peritoneal fluid was measured to quantify neutrophil recruitment. Zymosan-induced neutrophil recruitment was significantly greater (more than two-fold) in bile duct-ligated rats than in sham-ligated or normal animals. Depletion of peritoneal cells significantly suppressed neutrophil recruitment after zymosan injection in all three groups, with no significant differences between the groups. In normal rats, replacement of their peritoneal cells by those from bile duct-ligated rats did not enhance zymosan-induced neutrophil recruitment. In contrast, bile duct-ligated rats treated with peritoneal cell replacement from normals showed significantly increased neutrophil recruitment after zymosan injection. In vitro neutrophil chemotaxis in response to formyl-Met-Leu-Phe was significantly enhanced in bile duct-ligated rats, compared with that in sham-ligated animals. The results suggest that local neutrophil recruitment in response to inflammation may be enhanced in obstructive jaundice and that increased neutrophil chemotactic activity, not macrophage activity, may play a prime role in the mechanism.
在大鼠中研究了梗阻性黄疸对炎症刺激下局部中性粒细胞聚集的影响。通过胆管结扎7天造成梗阻性黄疸。腹腔注射酵母聚糖（200mg），4小时后测量腹腔液中的髓过氧化物酶活性以量化中性粒细胞募集。酵母聚糖诱导的中性粒细胞募集在胆管结扎大鼠中比假结扎或正常动物中显著增加（超过两倍）。在所有三组中，腹腔细胞耗竭显著抑制了酵母聚糖注射后的中性粒细胞募集，各组之间无显著差异。在正常大鼠中，用胆管结扎大鼠的腹腔细胞替代其自身的腹腔细胞并未增强酵母聚糖诱导的中性粒细胞募集。相反，用正常大鼠的腹腔细胞替代处理的胆管结扎大鼠在注射酵母聚糖后显示中性粒细胞募集显著增加。与假结扎动物相比，胆管结扎大鼠对甲酰甲硫氨酸亮氨酰苯丙氨酸的体外中性粒细胞趋化性显著增强。结果表明，梗阻性黄疸时对炎症的局部中性粒细胞募集可能增强，并且中性粒细胞趋化活性增加而非巨噬细胞活性可能在该机制中起主要作用。
