Yanagitani K, Kubota Y, Tsuji K, Yamamoto S, Amoh Y, Takaoka M, Ogura M, Inoue K
Third Department of Internal Medicine, Kansai Medical University, Moriguchi, Osaka, Japan.
J Gastroenterol. 1998 Aug;33(4):536-40. doi: 10.1007/s005350050128.
The effect of obstructive jaundice on neutrophil chemotactic function was investigated, with a potent chemotactic factor, IL-8 (recombinant rat GRO-beta), in rats that received 7-day bile duct ligation. Carrageenin or IL-8 was injected into a preformed air pouch, and exudate was collected 4 h later for measurement of myeloperoxidase activity. In vitro chemotaxis of peripheral neutrophils to IL-8 was evaluated by a modified Boyden chamber method. Both carrageenin and IL-8 induced significantly pronounced intra-air pouch neutrophil recruitment in the bile duct-ligated group compared with a sham-ligated group. In vitro neutrophil chemotaxis was significantly increased in the bile duct-ligated group compared with the sham-ligated group. The present experimental model suggests enhanced neutrophil chemotaxis to IL-8 in obstructive jaundice.
在接受7天胆管结扎的大鼠中，使用一种有效的趋化因子白细胞介素-8（重组大鼠GRO-β），研究阻塞性黄疸对中性粒细胞趋化功能的影响。将角叉菜胶或白细胞介素-8注入预先形成的气袋中，4小时后收集渗出液以测量髓过氧化物酶活性。通过改良的Boyden小室法评估外周血中性粒细胞对白细胞介素-8的体外趋化作用。与假结扎组相比，角叉菜胶和白细胞介素-8在胆管结扎组中均诱导气袋内中性粒细胞明显募集。与假结扎组相比，胆管结扎组的体外中性粒细胞趋化作用显著增强。本实验模型表明阻塞性黄疸中中性粒细胞对白细胞介素-8的趋化作用增强。
