Artico M, De Santis S, Cavallotti C
Chair of Human Anatomy, Faculty of Pharmacy, Department of Cardiovascular and Respiratory Sciences, University of Rome La Sapienza, Italy.
Cephalalgia. 1998 May;18(4):183-91. doi: 10.1046/j.1468-2982.1998.1804183.x.
The aim of the present study was to examine whether mast cells have the same variations as the related catecholaminergic nerve fibers. Chemical sympathectomy or surgical removal of right superior cervical ganglion induced a rapid decrease of fluorescence in both nerve fibers and mast cells, as confirmed by quantitative analysis (nerve fibers 19 +/- 1.1 vs 1.3 +/- 0.6; mast cell 10.8 +/- 1.9 vs 2.1 +/- 0.3). The results of quantitative analysis after nerve fiber stimulation (electrical), however, showed an increase of the fluorescence in both the nerve fibers and the mast cells (nerve fibers 43.4 +/- 2.4; mast cells 18.6 +/- 1.6). Moreover, we found that the basal zone is more innervated (regarding catecholaminergic nerve fibers) than the apical one, and that the fluorescence level decreases passing from the vasal zone to the perivasal and intervasal zones. Further studies are needed in order to clarify the role of fluorescent nerve fibers and mast cells of cerebral dura mater in cephalalgia.
本研究的目的是检验肥大细胞是否与相关的儿茶酚胺能神经纤维有相同的变化。化学性交感神经切除术或手术切除右侧颈上神经节导致神经纤维和肥大细胞中的荧光迅速减少，定量分析证实了这一点（神经纤维：19±1.1对1.3±0.6；肥大细胞：10.8±1.9对2.1±0.3）。然而，神经纤维刺激（电刺激）后的定量分析结果显示，神经纤维和肥大细胞中的荧光均增加（神经纤维：43.4±2.4；肥大细胞：18.6±1.6）。此外，我们发现，与顶端区域相比，基底区域（关于儿茶酚胺能神经纤维）的神经支配更多，并且从血管区域到血管周围和血管间区域，荧光水平降低。为了阐明硬脑膜中荧光神经纤维和肥大细胞在头痛中的作用，还需要进一步的研究。
