De Jong W W, Terwindt E C
Eur J Biochem. 1976 Aug 16;67(2):503-10. doi: 10.1111/j.1432-1033.1976.tb10716.x.
The amino acid sequence of the A chain of the eye lens protein alpha-crystallin from the red kangaroo (Macropus rufus) was completely determined by manual Edman degradation of tryptic, thermolytic and cyanogen bromide peptides. The sequence of the alpha-crystallin A chain from the Virginia opossum (Didelphis marsupialis) was deduced from amino acid analyses and partial Edman degradation of peptides. The 173-residue A chains of kangaroo and opossum differ in six positions, whereas comparison with the bovine alpha-crystallin A chain reveals 17 and 22 substitutions, respectively. Most substitutions occur in the COOH-terminal part of the chain.
通过对胰蛋白酶、嗜热菌蛋白酶和溴化氰肽段进行手动埃德曼降解，完全确定了红袋鼠（大赤袋鼠）晶状体蛋白α-晶体蛋白A链的氨基酸序列。根据氨基酸分析和肽段的部分埃德曼降解，推导了弗吉尼亚负鼠（北美负鼠）α-晶体蛋白A链的序列。袋鼠和负鼠的173个残基的A链在六个位置上不同，而与牛α-晶体蛋白A链相比，分别有17个和22个取代。大多数取代发生在链的COOH末端部分。
