Park J I, Yoshida I, Tada T, Takagi N, Takahashi Y, Kanagawa H
Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo.
Jpn J Vet Res. 1998 May;46(1):19-28.
The in vitro differentiative potential of mouse parthenogenetic (PG) embryonic stem (PGES) cells were investigated in the formation of embryoid bodies (EBs). EBs derived from PGES cells retarded in growth and showed restricted differentiation compared to their fertilized counterpart. In chimeric EBs from the aggregation of PGES and fertilized ES cells, morphological examination revealed that PGES cells were reduced in their population and distributed in endodermal layer as culture periods proceeded. These findings were comparable to those in aggregation chimeras of fertilized and PG embryos, and suggest that the differentiation of PGES cells in vitro is restricted in the formation of EBs.
研究了小鼠孤雌生殖（PG）胚胎干细胞（PGES细胞）在体外形成拟胚体（EBs）的分化潜能。与受精胚胎干细胞衍生的EBs相比，PGES细胞衍生的EBs生长迟缓且分化受限。在PGES细胞与受精胚胎干细胞聚集形成的嵌合EBs中，形态学检查显示随着培养时间的推移，PGES细胞数量减少并分布在内胚层。这些发现与受精胚胎和PG胚胎聚集嵌合体中的发现相似，表明PGES细胞在体外形成EBs的过程中分化受限。
