Park J I, Yoshida I, Tada T, Takagi N, Takahashi Y, Kanagawa H
Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo.
Jpn J Vet Res. 1998 May;46(1):29-35.
Murine parthenogenetic embryonic stem (ES) cell lines expressing lac Z reporter gene were isolated after co-transfection with lac Z reporter gene (pENL) and neo gene (pSTneo) to TMA-48P cell line of 129/Sv origin. Karyotype analyses showed that all of four transfected cell lines examined contained 41 chromosomes with trisomy 8. Bacterial neo transgene required for G418 selection were integrated into several chromosomes including chromosome 8. Histological studies of teratomas formed in syngenic mice and embryoid bodies grown in vitro showed that the differentiative potential remained almost identical in chromosomally normal parental cell line and its derivative cell lines trisomic for chromosome 8.
将携带lacZ报告基因（pENL）和新霉素基因（pSTneo）共转染至源于129/Sv的TMA - 48P细胞系后，分离出表达lacZ报告基因的小鼠孤雌生殖胚胎干细胞（ES）系。核型分析显示，所检测的4个转染细胞系均含有41条染色体，伴有8号染色体三体。用于G418筛选的细菌新霉素转基因整合到了包括8号染色体在内的几条染色体上。对同基因小鼠体内形成的畸胎瘤以及体外培养的类胚体进行的组织学研究表明，染色体正常的亲代细胞系及其8号染色体三体的衍生细胞系的分化潜能几乎相同。
