幽门螺杆菌热休克蛋白60（HSP60）在幽门螺杆菌黏附人胃上皮细胞中的作用

[The role of heat shock protein 60 (HSP60) of Helicobacter pylori in adhesion of H. pylori to human gastric epithelial cell].

作者信息

Yamaguchi H, Osaki T, Kurihara N, Taguchi H, Kamiya S

机构信息

Department of Microbiology, Kyorin University School of Medicine.

出版信息

Kansenshogaku Zasshi. 1998 May;72(5):487-92. doi: 10.11150/kansenshogakuzasshi1970.72.487.

DOI:10.11150/kansenshogakuzasshi1970.72.487

PMID:9642937

Abstract

Adhesion of Helicobacter pylori to both human gastric carcinoma cell lines (MKN45, MKN28 and KATO III) and prepared primary human gastric epithelial cells were analyzed with flow-cytometry. All strains adhered to human gastric carcinoma cells. Especially, these strains strongly adhered to MKN45 cells. Adhesion of H. pylori strains to prepared primary human gastric epithelial cells was also observed. However, the adherence rates of H. pylori to these cells were different among the cells used. These results suggested that the host factor might be important for adhesion of the bacteria to human gasgric cells. In addition, H20 monoclonal antibody directed to H. pylori HSP60 inhibited the adhesion of H. pylori to both cells. These results indicate that H. pylori HSP60 might be associated with the adhesion of the bacteria to human gastric epithelial cells.

摘要

采用流式细胞术分析幽门螺杆菌对人胃癌细胞系（MKN45、MKN28和KATO III）以及制备的原代人胃上皮细胞的黏附情况。所有菌株均能黏附于人胃癌细胞。特别是，这些菌株强烈黏附于MKN45细胞。也观察到幽门螺杆菌菌株对制备的原代人胃上皮细胞的黏附。然而，幽门螺杆菌对这些细胞的黏附率在所用细胞之间存在差异。这些结果表明宿主因素可能对细菌黏附于人胃细胞很重要。此外，针对幽门螺杆菌HSP60的H20单克隆抗体抑制了幽门螺杆菌对两种细胞的黏附。这些结果表明幽门螺杆菌HSP60可能与人胃上皮细胞的细菌黏附有关。