一种抑制幽门螺杆菌黏附胃上皮细胞的单克隆抗体的建立与鉴定

Establishment and characterisation of a monoclonal antibody to inhibit adhesion of Helicobacter pylori to gastric epithelial cells.

作者信息

Osaki T, Yamaguchi H, Taguchi H, Fukuda M, Kawakami H, Hirano H, Watanabe S, Takagi A, Kamiya S

机构信息

Division of Flow Cytometry, Kyorin University School of Medicine, Shinkawa, Mitaka, Tokyo, Japan.

出版信息

J Med Microbiol. 1998 Jun;47(6):505-12. doi: 10.1099/00222615-47-6-505.

DOI:10.1099/00222615-47-6-505

PMID:9879969

Abstract

Monoclonal antibodies (MAbs) that inhibit adhesion of Helicobacter pylori to human gastric cancer (MKN45) cells were established to clarify the mechanism of adhesion of H. pylori. Of 53 hybridoma clones screened by the primary inhibition assay for adhesion, MAb A20 of IgM class was selected on the basis of both its reactivity to whole cells of H. pylori by ELISA and its inhibitory effect on adhesion of H. pylori. The adhesion of H. pylori strain TK1029 to MKN45 cells was inhibited by MAb A20, depending on the concentration of the MAb. The MAb recognised the surface antigen, lipopolysaccharide (LPS) of H. pylori, suggesting that LPS is associated with adhesion of H. pylori to human gastric epithelial cells.

摘要

为阐明幽门螺杆菌的黏附机制，制备了抑制幽门螺杆菌与人胃癌（MKN45）细胞黏附的单克隆抗体（MAb）。在通过黏附的初步抑制试验筛选的53个杂交瘤克隆中，IgM类单克隆抗体A20是基于其通过酶联免疫吸附测定法（ELISA）对幽门螺杆菌全细胞的反应性及其对幽门螺杆菌黏附的抑制作用而选择的。单克隆抗体A20可抑制幽门螺杆菌TK1029菌株对MKN45细胞的黏附，这取决于单克隆抗体的浓度。该单克隆抗体识别幽门螺杆菌的表面抗原脂多糖（LPS），表明LPS与幽门螺杆菌对人胃上皮细胞的黏附有关。