评估两种完全基于细菌DNA和rRNA扩增技术的商用结核分枝杆菌诊断试剂盒，用于直接检测痰标本中的结核杆菌。

Evaluation of two commercial diagnostic kits for Mycobacterium tuberculosis completely based on bacterial DNA and rRNA amplification for direct detection of tubercle bacilli in sputum specimens.

作者信息

Sato K, Tomioka H, Kawahara S, Shishido S

机构信息

Department of Microbiology and Immunology, Shimane Medical University, Japan.

出版信息

Kansenshogaku Zasshi. 1998 May;72(5):504-11. doi: 10.11150/kansenshogakuzasshi1970.72.504.

DOI:10.11150/kansenshogakuzasshi1970.72.504

PMID:9642940

Abstract

The Roche Amplicor Mycobacterium Tuberculosis Kit (Amplicor PCR) and Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test (MTD) were compared for efficiency in the direct detection of tubercle bacilli in sputum specimens. In total, 72 sputum specimens obtained from patients with active tuberculosis were examined with the Amplicor PCR and MTD kits. Of the 72 test specimens, 45 and 41 samples were positive by the Amplicor PCR and MTD methods, respectively. These values were larger than those obtained by the culture method (Septi-Chek) and acid-fast bacilli-smear test, yielding 37 and 20 positive results, respectively, and the sensitivities were as follows: smear, 39.2%; culture, 72.5%; Amplicor PCR, 88.2%; MTD, 80.4%. Amplicor PCR was similarly or somewhat more sensitive in direct detection of M. tuberculosis in sputum samples from patients with clinically active tuberculosis than was MTD.

摘要

对罗氏Amplicor结核分枝杆菌检测试剂盒（Amplicor PCR）和基因探针扩增结核分枝杆菌直接检测法（MTD）在痰液标本中直接检测结核杆菌的效率进行了比较。总共用Amplicor PCR和MTD试剂盒检测了72份来自活动性肺结核患者的痰液标本。在这72份检测标本中，Amplicor PCR法和MTD法分别有45份和41份样本呈阳性。这些数值高于通过培养法（Septi-Chek）和抗酸杆菌涂片检测法得到的数值，培养法和抗酸杆菌涂片检测法分别得到37份和20份阳性结果，敏感性如下：涂片，39.2%；培养，72.5%；Amplicor PCR，88.2%；MTD，80.4%。在直接检测临床活动性肺结核患者痰液样本中的结核分枝杆菌方面，Amplicor PCR与MTD同样敏感或略更敏感。