Ogura Y, Sato K, Kato T, Saito K, Enomoto K
Department of Urology, School of Medicine, Akita University.
Nihon Hinyokika Gakkai Zasshi. 1998 May;89(5):529-37. doi: 10.5980/jpnjurol1989.89.529.
We evaluated expression of angiogenic factors, tumor vessel density and recurrence in superficial bladder cancer by immunohistochemistry.
Fourty tumor specimens from bladder preserving operation and 11 normal epithelia were stained for factor VIII-related antigen, vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and acidic fibroblast growth factor (aFGF). Maximum vessel density (MVD) was counted in a 200x field in the highest vascularized area stained for factor VIII. Superimposing a 4 x 4 square ocular grid on the boundary between epithelium and interstitium, average vessel density (AVD) and vascular surface density (VSD) were calculated by dividing the vessel number in the grid and the intersection number of vessel walls on the lattice lines by the area of interstitium calculated from the number of the gird cross points, respectively.
Positive rates of VEGF, bFGF and aFGF by immunohistochemistry were 50%, 23% and 43% in the superficial bladder cancer, respectively. The positive rate of VEGF staining was higher in G2-3 tumors than in G1 tumors. All normal epithelia, except one which was positive for aFGF, was negative for those three factors. T1 tumors had a higher MVD than that of Ta. AVD and VSD of the tumor were higher than those of normal epithelium, but negative correlation between the number of positive angiogenic factors and the vessel density was revealed. The recurrence group had a lower vessel density and a higher ratio of VSD/AVD than those of the non-recurrence group.
These findings suggest that the expression of VEGF, bFGF and aFGF was not reflected by the tumor angiogenesis, the angiogenesis is required topically at the invasive front of the superficial bladder cancer, and the tumor which had fewer and broader tumor vessels has a high tendency to recur.
我们通过免疫组织化学评估浅表性膀胱癌中血管生成因子的表达、肿瘤血管密度及复发情况。
对40例膀胱保留手术的肿瘤标本及11例正常上皮组织进行因子VIII相关抗原、血管内皮生长因子（VEGF）、碱性成纤维细胞生长因子（bFGF）和酸性成纤维细胞生长因子（aFGF）染色。在因子VIII染色的血管化程度最高区域的200倍视野下计数最大血管密度（MVD）。在上皮与间质边界叠加4×4方形目镜网格，分别通过网格内血管数量及网格线上血管壁交叉点数除以由网格交叉点数量计算出的间质面积来计算平均血管密度（AVD）和血管表面密度（VSD）。
浅表性膀胱癌中VEGF、bFGF和aFGF免疫组织化学阳性率分别为50%、23%和43%。G2 - 3期肿瘤VEGF染色阳性率高于G1期肿瘤。除1例aFGF阳性的正常上皮组织外，其余所有正常上皮组织这三种因子均为阴性。T1期肿瘤的MVD高于Ta期肿瘤。肿瘤的AVD和VSD高于正常上皮组织，但血管生成因子阳性数量与血管密度呈负相关。复发组的血管密度低于非复发组，VSD/AVD比值高于非复发组。
这些发现表明VEGF、bFGF和aFGF的表达未反映在肿瘤血管生成中，浅表性膀胱癌侵袭前沿局部需要血管生成，且肿瘤血管较少且较宽的肿瘤复发倾向较高。
