Jasin H E
Clin Exp Immunol. 1975 Dec;22(3):473-85.
The role of acute inflammation and of pre-existing specific antibody in the retention of intra-articular antigen in joint collagenous tissues of immunized rabbits was examined. The role of the acute synovitis occurring immediately after antigen injection was investigated by the production of acute synovitis in immune and non-immune rabbits. In no case was more 125I-labelled BSA retained in the inflamed joint tissues compared to the contralateral non-inflamed joints 7 days after intrarticular antigen injection. When antigen retention was examined early after intra-articular injection, the largest amount of antigen was retained 30 min after injection, before the appearance of the acute inflammatory synovitis. These findings suggest that acute inflammation does not constitute a major factor in the long-term retention of antigen in collagenous tissues. To investigate the role of antibody in the retention of antigen, non-immune rabbits were injected intravenously with purified anti-BSA antibody 3 days prior to the intra-articular injection of BSA. Over 20 times more antigen was retained irreversibly in collagenous tissues obtained from the injected joints of passively immunized animals compared with similar tissues of control rabbits. When rabbits were injected intravenously with purified anti-BSA antibody and either killed 20 mins or 3 days later, in vitro binding of antigen by joint collagenous tissues was seen only in animals where antibody was allowed to equilibrate with the extravascular space for 3 days. These findings indicate that retention of antigen depends on the presence of extravascular antibody. Antigen retention in collagenous tissues was also present when both antibody and antigen were injected intravenously and antibody was given 3 days before the antigen. It is concluded that the trapping of immune complexes in collagenous joint tissues of immunized animals depends on: the presence of antibody in the extra-vascular space; the diffusion of antigen or soluble complexes into this space; the interaction of antigen or soluble complexes with extravascular antibody with subsequent formation of larger and more insoluble complexes; and the trapping of these complexes within the collagen fibre meshwork.
研究了急性炎症和预先存在的特异性抗体在免疫兔关节胶原组织中关节内抗原滞留方面的作用。通过在免疫和非免疫兔中引发急性滑膜炎，研究了抗原注射后立即发生的急性滑膜炎的作用。关节内注射抗原7天后，与对侧未发炎关节相比，发炎关节组织中保留的125I标记牛血清白蛋白（BSA）在任何情况下都不超过125I标记牛血清白蛋白（BSA）。当在关节内注射后早期检查抗原滞留情况时，注射后30分钟，在急性炎症性滑膜炎出现之前，抗原的保留量最大。这些发现表明，急性炎症并非抗原在胶原组织中长期滞留的主要因素。为了研究抗体在抗原滞留中的作用，在关节内注射BSA前3天，给非免疫兔静脉注射纯化的抗BSA抗体。与对照兔的相似组织相比，从被动免疫动物的注射关节获得的胶原组织中不可逆保留的抗原多20倍以上。当给兔静脉注射纯化的抗BSA抗体，并在20分钟或3天后处死时，仅在抗体与血管外空间平衡3天的动物中观察到关节胶原组织对抗原的体外结合。这些发现表明，抗原的滞留取决于血管外抗体的存在。当抗体和抗原都静脉注射且抗体在抗原前3天给予时，胶原组织中也存在抗原滞留。得出的结论是，免疫动物胶原关节组织中免疫复合物的捕获取决于：血管外空间中抗体的存在；抗原或可溶性复合物扩散到该空间；抗原或可溶性复合物与血管外抗体相互作用，随后形成更大且更不溶性的复合物；以及这些复合物在胶原纤维网内的捕获。
