Hoyer L L, Payne T L, Bell M, Myers A M, Scherer S
Department of Veterinary Pathobiology, University of Illinois at Urbana-Champaign, 2522 VMBSB, 2001 S. Lincoln Avenue, Urbana, IL 61802, USA.
Curr Genet. 1998 Jun;33(6):451-9. doi: 10.1007/s002940050359.
The ALS1 (agglutinin-like sequence) gene of Candida albicans encodes a protein similar to alpha-agglutinin, a cell-surface adhesion glycoprotein of Saccharomyces cerevisiae (Hoyer et al. 1995). A central domain of a tandemly repeated 108-bp sequence is found in the ALS1 coding region. This tandem-repeat motif hybridizes to multiple C. albicans genomic DNA fragments, indicating the possibility of other ALS1-like genes in C. albicans (Hoyer et al. 1995). To determine if these fragments constitute a gene family, tandem-repeat-hybridizing genomic fragments were isolated from a fosmid library by PCR screening using primers based on the consensus tandem-repeat sequence of ALS1 (Hoyer et al. 1995). One group of fosmids, designated ALS3, encodes a gene with 81% identity to ALS1. The sequences of ALS1 and ALS3 are most conserved in the tandem-repeat domain and in the region 5' of the tandem repeats. Northern-blot analysis using unique probes from the 3' end of each gene demonstrated that ALS1 expression varies, depending on which C. albicans strain is examined, and that ALS3 is hyphal-specific. Both genes are found in a variety of C. albicans and C. stellatoidea strains examined. The predicted Als1p and Als3p exhibit features suggesting that both are cell-surface glycoproteins. Southern blots probed with conserved sequences from the region 5' of the tandem repeats suggest that other ALS-like sequences are present in the C. albicans genome and that the ALS family may be larger than originally estimated.
白色念珠菌的ALS1（凝集素样序列）基因编码一种与α-凝集素相似的蛋白质，α-凝集素是酿酒酵母的一种细胞表面粘附糖蛋白（霍耶等人，1995年）。在ALS1编码区发现了一个由108个碱基对串联重复序列组成的中央结构域。这个串联重复基序与多个白色念珠菌基因组DNA片段杂交，表明白色念珠菌中可能存在其他ALS1样基因（霍耶等人，1995年）。为了确定这些片段是否构成一个基因家族，通过基于ALS1共有串联重复序列的引物进行PCR筛选，从fosmid文库中分离出串联重复杂交的基因组片段（霍耶等人，1995年）。一组名为ALS3的fosmid编码一个与ALS1具有81%同一性的基因。ALS1和ALS3的序列在串联重复结构域和串联重复序列5'端区域最为保守。使用来自每个基因3'端的独特探针进行的Northern印迹分析表明，ALS1的表达因所检测的白色念珠菌菌株而异，并且ALS3是菌丝特异性的。在所检测的各种白色念珠菌和星状念珠菌菌株中都发现了这两个基因。预测的Als1p和Als3p表现出表明两者都是细胞表面糖蛋白的特征。用串联重复序列5'端区域的保守序列进行Southern印迹杂交表明，白色念珠菌基因组中存在其他ALS样序列，并且ALS家族可能比最初估计的更大。
