Czucz Varga Jarmila, Bujdák Juraj, Bujdáková Helena
Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina, Ilkovičova 6, 842 15 Bratislava, Slovakia.
Department of Physical and Theoretical Chemistry, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina, Ilkovičova 6, 842 15 Bratislava, Slovakia.
J Fungi (Basel). 2025 Aug 5;11(8):582. doi: 10.3390/jof11080582.
and are opportunistic pathogens that cause life-threatening infections. This study focused on using photodynamic inactivation (PDI) to eliminate mixed biofilms of formed on poly (urethane) (PU) discs functionalized with a nanocomposite layer containing phloxine B (PhB). Additionally, the effect of PDI on the and genes of was examined in mixed biofilms. Spectral analysis showed a continuous release of PhB from the nanocomposite in Mueller-Hinton broth within 48 h, with a released amount of PhB < 5% of the total amount. The anti-biofilm effectiveness of the light-activated nanocomposite with PhB showed a reduction in the survival rate of biofilm cells to 0.35% and 31.79% for and , respectively, compared to the control biofilm on PU alone. Scanning electron microscopy images showed that the nanocomposite effectively reduced the colonization and growth of the mixed biofilm. While PDI reduced the regulation of the gene, the gene was upregulated. Nevertheless, the cell survival of the - biofilm was significantly reduced, showing great potential for the elimination of mixed biofilms.
[具体病原体名称1]和[具体病原体名称2]是可引发危及生命感染的机会致病菌。本研究聚焦于利用光动力灭活（PDI）来消除在含有玫瑰红B（PhB）的纳米复合层功能化的聚（聚氨酯）（PU）圆盘上形成的[具体病原体名称1]和[具体病原体名称2]的混合生物膜。此外，在混合生物膜中检测了PDI对[具体病原体名称1]的[具体基因1]和[具体基因2]基因的影响。光谱分析表明，在48小时内，纳米复合物中的PhB在 Mueller-Hinton肉汤中持续释放，PhB释放量 < 总量的5%。与仅在PU上的对照生物膜相比，含PhB的光激活纳米复合物的抗生物膜有效性显示生物膜细胞存活率分别降至[具体病原体名称1]的0.35%和[具体病原体名称2]的31.79%。扫描电子显微镜图像显示，纳米复合物有效减少了混合生物膜的定植和生长。虽然PDI降低了[具体基因1]基因的调控，但[具体基因2]基因被上调。尽管如此，[具体病原体名称1] -生物膜的细胞存活率显著降低，显示出消除混合生物膜的巨大潜力。
