Dubik E P, Treus V V, Nikitin N S, Smirnov A F
All-Russian Research Institute of Genetics and Farm Animal Breeding, St. Petersburg--Pushkin.
Tsitologiia. 1998;40(4):317-22.
Chromosomal aberrations (CAs), sister-chromatid exchanges (SCEs), aneuploidy and proliferative potential (PP) were investigated in peripheral blood lymphocytes of healthy cows (control group-C), BLV-(bovine leucosis virus)-infected cattle without hematological abnormalities (RID--seropositive group (I) and affected with leucaemia (lymphocytosis (LC), lymphoma (L)). Nonrandom chromosomal (marker) aberrations were not found in the cow group at stage LC. The levels of aneuploidy and SCEs increased in the cow group at stage L compared to the cow group at stage I. Polyploidy: C--1.9 +/- 0.28, I--3.5 +/- 0.22, LC--6.1 +/- 0.82, L--10.5 +/- 0.51 (P < 0.01). Hypoploidy (2n = 58): C--3.0 +/- 0.17, I--54 +/- 0.71, LC--12.1 +/- 0.72, L--14.0 +/- 0.65 (P < 0.01). SCEs: C--3.8 +/- 0.26, I--5.4 +/- 0.15, LC--7.2 +/- 0.16, L--9.7 +/- 0.26 (P < 0.01). There are no differences in CAs rates and PP between groups of cows at all the observed stages of leucaemic process. The obtained results are discussed in terms of cytogenetic aspects of leucaemic process in cows.
对健康奶牛（对照组 - C）、感染牛白血病病毒（BLV）但无血液学异常的牛（RID - 血清阳性组（I））以及患白血病（淋巴细胞增多症（LC）、淋巴瘤（L））的牛的外周血淋巴细胞进行了染色体畸变（CA）、姐妹染色单体交换（SCE）、非整倍体和增殖潜力（PP）的研究。在淋巴细胞增多症阶段的奶牛组中未发现非随机染色体（标记）畸变。与I期奶牛组相比，L期奶牛组的非整倍体和SCE水平升高。多倍体：C组 - 1.9 ± 0.28，I组 - 3.5 ± 0.22，LC组 - 6.1 ± 0.82，L组 - 10.5 ± 0.51（P < 0.01）。亚二倍体（2n = 58）：C组 - 3.0 ± 0.17，I组 - 54 ± 0.71，LC组 - 12.1 ± 0.72，L组 - 14.0 ± 0.65（P < 0.01）。SCE：C组 - 3.8 ± 0.26，I组 - 5.4 ± 0.15，LC组 - 7.2 ± 0.16，L组 - 9.7 ± 0.26（P < 0.01）。在白血病过程的所有观察阶段，奶牛组之间的CA发生率和PP没有差异。从奶牛白血病过程的细胞遗传学方面对所得结果进行了讨论。
