Campbell R E, Lu W, White A K, Duhamel G E
Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln, USA.
Arch Virol. 1998;143(5):1021-8. doi: 10.1007/s007050050351.
Bovine rotavirus strain B223 is the North American prototype for group A P8[11] serotype/genotype rotaviruses. Rotaviruses with this serotype/genotype are a prevalent cause of neonatal calf diarrhea and have also been isolated from asymptomatic human infants. On the basis of deduced amino acid sequence of the outer capsid viral protein 4 (VP4), strain B223 lacks a cysteine at position 318 that is conserved among all other rotavirus strains. It has been speculated that this may result in the loss of disulfide bond and a change in the structure of the VP4 that may affect the infectivity and antigenicity of the virus. This paper describes partial sequences of the VP4 gene (nucleotides 613 to 1016 coding for amino acid positions 202 to 335) of 16 bovine rotavirus field isolates with P8[11] that were obtained from calves in Nebraska and Indiana. All the isolates lack a cystein at position 203 and had a conserved valine residue at position 318, thus indicating that the prototype strain B223 is representative of group A rotaviruses with P8[11] serotype/genotype.
牛轮状病毒B223株是A组P8[11]血清型/基因型轮状病毒的北美原型。具有这种血清型/基因型的轮状病毒是新生犊牛腹泻的常见病因，也已从无症状人类婴儿中分离出来。根据外衣壳病毒蛋白4（VP4）的推导氨基酸序列，B223株在318位缺少一个半胱氨酸，而在所有其他轮状病毒株中该位置是保守的。据推测，这可能导致二硫键的丧失以及VP4结构的改变，进而可能影响病毒的感染性和抗原性。本文描述了从内布拉斯加州和印第安纳州的犊牛中获得的16株具有P8[11]的牛轮状病毒田间分离株的VP4基因部分序列（编码氨基酸位置202至335的核苷酸613至1016）。所有分离株在203位均缺少一个半胱氨酸，并且在318位有一个保守的缬氨酸残基，因此表明原型株B223代表具有P8[11]血清型/基因型的A组轮状病毒。
