Mummidi S, Brooks M A, Paul P S, Lyoo Y S, Zaberezhny A D
Veterinary Medical Research Institute, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
Vet Microbiol. 1996 Aug;51(3-4):241-55. doi: 10.1016/0378-1135(96)00050-8.
We have previously reported the isolation of a bovine rotavirus, designated VMRI, with a super-short electropherotype. We have characterized this strain further as it has shown antigenic differences with the prototype G6 strain NCDV-Lincoln. In this communication, we report the antigenic and molecular characterization and the nucleotide sequence of the VP4 and VP7 genes of this strain. Virus neutralization tests indicated 2- to 13-fold differences in the titers between NCDV-Lincoln, B641 and VMRI strains. Northern blot hybridization results indicated a degree of heterogeneity in the VP4 gene of these strains which can be detected under conditions of high stringency. The VP4 and VP7 genes of the VMRI strain were cloned and sequenced and compared with the published sequences of other bovine rotavirus strains. The VP4 gene of VMRI had a high degree of homology with that of UK and B641 strains but differed significantly from that of both NCDV-Lincoln and B223 strains. Sequence analysis of the VP7 gene of VMRI and other strains indicated a high degree of conservation and the amino acid identity between the different strains was 96%. Sequence information regarding these strains and field isolates will assist in the generation of effective vaccination strategies for control of neonatal calf diarrhea.
我们之前报道过分离出一种具有超短电泳型的牛轮状病毒，命名为VMRI。由于该毒株与原型G6毒株NCDV-Lincoln表现出抗原差异，我们对其进行了进一步鉴定。在本通讯中，我们报道了该毒株VP4和VP7基因的抗原性和分子特征以及核苷酸序列。病毒中和试验表明，NCDV-Lincoln、B641和VMRI毒株之间的滴度差异为2至13倍。Northern印迹杂交结果表明，在高严格条件下可检测到这些毒株VP4基因存在一定程度的异质性。克隆并测序了VMRI毒株的VP4和VP7基因，并与其他牛轮状病毒毒株的已发表序列进行了比较。VMRI的VP4基因与英国和B641毒株的VP4基因具有高度同源性，但与NCDV-Lincoln和B223毒株的VP4基因有显著差异。VMRI和其他毒株VP7基因的序列分析表明其具有高度保守性，不同毒株之间的氨基酸同一性为96%。有关这些毒株和田间分离株的序列信息将有助于制定控制新生犊牛腹泻的有效疫苗接种策略。
