Turner K S, Kleven S H
Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602-4875, USA.
Avian Dis. 1998 Apr-Jun;42(2):404-7.
Subsequent to use of a live Mycoplasma gallisepticum (MG) vaccination program, the F strain of MG had been circulating on a commercial layer farm since 1981. In 1994, the ts-11 strain was introduced on the farm; each new placement flock was vaccinated by eyedrop with ts-11 for one production cycle, and then all subsequent placement flocks were left unvaccinated. Birds were monitored by culture and serology before and after vaccination. MG isolates were characterized by random amplified polymorphic DNA (RAPD). MG was isolated from ts-11-vaccinated flocks up to 100 wk of age; all such isolates tested by RAPD were ts-11 type. After ts-11 vaccination was discontinued, no MG was detected in nonvaccinated birds. After the last vaccinated flock was marketed, no MG was detected on the farm. These results indicate a potential use for ts-11 in an MG eradication program.
在使用活的鸡毒支原体（MG）疫苗接种程序之后，自1981年以来，MG的F株一直在一个商业蛋鸡养殖场中传播。1994年，ts-11株被引入该养殖场；每个新入栏鸡群在一个生产周期内通过滴眼接种ts-11疫苗，之后所有后续入栏鸡群不再接种。在接种前后通过培养和血清学对鸡进行监测。通过随机扩增多态性DNA（RAPD）对MG分离株进行鉴定。在高达100周龄的接种ts-11疫苗的鸡群中分离到MG；所有通过RAPD检测的此类分离株均为ts-11型。在停止接种ts-11疫苗后，在未接种疫苗的鸡中未检测到MG。在最后一批接种疫苗的鸡群上市后，在该养殖场未检测到MG。这些结果表明ts-11在MG根除计划中有潜在用途。
