Rossi V, Hartings H, Motto M
Istituto Sperimentale per la Ceralicoltura, Sezione di Bergamo, Italy.
Mol Gen Genet. 1998 May;258(3):288-96. doi: 10.1007/s004380050733.
In mammals, yeast and Drosophila, the histone deacetylase RPD3 proteins can alter the expression of genes involved in fundamental biological processes by affecting the degree of acetylation of histones and changing chromatin structure. Here we report the isolation of a cDNA sequence encoding an RPD3 homologue from maize, which is able to complement the phenotype of an rpd3 null mutant of the yeast Saccharomyces cerevisiae. The expression of the corresponding gene(s) was assessed in different maize tissues. The number of homologous loci was estimated by Southern hybridisation to be in the range of two to three, and the chromosomal location of one of these loci was determined. Phylogenetic analysis and tests for relative divergence rates, using related RPD3 sequences from different species, were performed, and suggest that different polymorphic forms of RPD3-like proteins that evolve at distinct rates are present in the species considered.
在哺乳动物、酵母和果蝇中，组蛋白去乙酰化酶RPD3蛋白可通过影响组蛋白的乙酰化程度和改变染色质结构，来改变参与基本生物学过程的基因的表达。本文我们报道了从玉米中分离出一个编码RPD3同源物的cDNA序列，该序列能够弥补酿酒酵母rpd3缺失突变体的表型。在不同的玉米组织中评估了相应基因的表达。通过Southern杂交估计同源基因座的数量在两到三个范围内，并确定了其中一个基因座的染色体位置。利用来自不同物种的相关RPD3序列进行了系统发育分析和相对分化率测试，结果表明在所研究的物种中存在以不同速率进化的不同多态形式的RPD3样蛋白。
