Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China.
Int J Mol Sci. 2022 May 25;23(11):5957. doi: 10.3390/ijms23115957.
Histone deacetylases (HDACs) play important roles in plant growth, development, and stress response. However, the pattern of how they are expressed in response to cold stress in the ornamental woody plant is poorly understood. Here, we identify 121 from eight Rosaceae plants of which 13 genes are from . A phylogenetic analysis suggests that the family is classified into three subfamilies, , and . We identify 11 segmental duplication gene pairs of and find, via a sequence alignment, that the gene family, especially the plant-specific family, has experienced gene expansion and contraction at a recent genome evolution history. Each of the three subfamilies has its own conserved domains. The expression of in mei is found to be tissue-specific or tissue-wide. RNA-seq data and qRT-PCR experiments in cold treatments suggest that almost all genes-especially , , and -significantly respond to cold stress. Our analysis provides a fundamental insight into the phylogenetic relationship of the family in Rosaceae plants. Expression profiles of in response to cold stress could provide an important clue to improve the cold hardiness of mei.
组蛋白去乙酰化酶（HDACs）在植物的生长、发育和应激反应中发挥着重要作用。然而，它们在观赏木本植物中对冷胁迫的响应模式还知之甚少。在这里，我们从八种蔷薇科植物中鉴定出 121 个，其中 13 个基因来自。系统发育分析表明，该家族分为三个亚家族，、和。我们鉴定了 11 对和的串联重复基因对，并通过序列比对发现，基因家族，特别是植物特异性家族，在最近的基因组进化历史中经历了基因扩张和收缩。三个亚家族都有自己的保守结构域。在梅中发现 基因的表达具有组织特异性或组织广泛性。RNA-seq 数据和冷处理的 qRT-PCR 实验表明，几乎所有的 基因——尤其是 、和——都显著响应冷胁迫。我们的分析为蔷薇科植物中 家族的系统发育关系提供了基本的认识。对冷胁迫响应的 基因表达谱可能为提高梅的抗寒性提供重要线索。
