Kim J H, Ogawa K, Wakabayashi H
Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
J Parasitol. 1998 Jun;84(3):552-6.
The respiratory burst assay was conducted using the nitroblue tetrazolium reduction method and horseradish peroxidase method to investigate the events when ayu head kidney macrophages phagocytize fresh and formalin-killed Glugea plecoglossi spores. The production of O2 against G. plecoglossi spores was negligible compared to zymosan (P < 0.01). Zymosan-induced O2 production was markedly inhibited by adding G. plecoglossi spores simultaneously (P < 0.01). This phenomenon was dose dependent, and killed spores were less inhibitory than fresh spores. The production of H2O2 was drastically increased when G. plecoglossi spores were added (P < 0.01), and most spores were phagocytized. From these results, it is suggested that G. plecoglossi spores modulate the host's phagocytic response to establish infection.
采用硝基蓝四氮唑还原法和辣根过氧化物酶法进行呼吸爆发试验，以研究香鱼头肾巨噬细胞吞噬新鲜和福尔马林灭活的鲈嗜碘孢虫孢子时的情况。与酵母聚糖相比，针对鲈嗜碘孢虫孢子产生的O₂可忽略不计（P < 0.01）。同时添加鲈嗜碘孢虫孢子可显著抑制酵母聚糖诱导的O₂产生（P < 0.01）。这种现象呈剂量依赖性，灭活孢子的抑制作用小于新鲜孢子。添加鲈嗜碘孢虫孢子时，H₂O₂的产生急剧增加（P < 0.01），且大多数孢子被吞噬。从这些结果表明，鲈嗜碘孢虫孢子调节宿主的吞噬反应以建立感染。
