Pagani A, Macrí L, Rosolen A, Toffolatti L, Stella A, Bussolati G
Department of Biomedical Sciences and Human Oncology, University of Torino, Italy.
Diagn Mol Pathol. 1998 Feb;7(1):36-43. doi: 10.1097/00019606-199802000-00007.
Ewing's sarcomas (ESs), primitive neuroectodermal tumors (PNETs), and neuroblastomas (NBs) are closely related neoplasms supposedly derived from the neural crest and belonging to the family of the small blue round cell tumors of infancy and childhood. We investigated the expression of the neuroendocrine and neuroectodermal markers chromogranin A (CgA) and secretogranin II (SgII) in ESs, PNETs, and NBs, both in primitive tumors (five, nine, and four cases, respectively) and in established cell lines (three ES and two PNET cell lines). Different technical approaches, namely immunohistochemistry, Northern blot analysis, and reverse transcriptase-polymerase chain reaction (RT-PCR) were used in parallel. Chromogranin A and secretogranin II production was constantly detectable in NBs by all procedures. CgA mRNA was detectable in most ESs and PNETs only by RT-PCR, whereas SgII mRNA was detectable in some ESs and PNETs by Northern blot analysis and in all tumors by RT-PCR. CgA and SgII proteins were never detectable by immunohistochemistry in ESs and PNETs. We conclude that neuroendocrine differentiation is shared by all three tumor entities, being more overt in NBs and rudimentary in ESs and PNETs; traces of chromogranin mRNA are detectable only by a highly sensitive RT-PCR procedure.
尤因肉瘤（ES）、原始神经外胚层肿瘤（PNET）和神经母细胞瘤（NB）是密切相关的肿瘤，推测起源于神经嵴，属于婴幼儿和儿童期小蓝圆细胞肿瘤家族。我们研究了神经内分泌和神经外胚层标志物嗜铬粒蛋白A（CgA）和分泌粒蛋白II（SgII）在ES、PNET和NB中的表达，包括原始肿瘤（分别为5例、9例和4例）和已建立的细胞系（3个ES细胞系和2个PNET细胞系）。同时采用了不同的技术方法，即免疫组织化学、Northern印迹分析和逆转录聚合酶链反应（RT-PCR）。通过所有检测程序在NB中均能持续检测到嗜铬粒蛋白A和分泌粒蛋白II的产生。仅通过RT-PCR在大多数ES和PNET中可检测到CgA mRNA，而通过Northern印迹分析在一些ES和PNET中可检测到SgII mRNA，通过RT-PCR在所有肿瘤中均可检测到。通过免疫组织化学在ES和PNET中从未检测到CgA和SgII蛋白。我们得出结论，这三种肿瘤实体均存在神经内分泌分化，在NB中更为明显，在ES和PNET中则较为初级；仅通过高度敏感的RT-PCR程序才能检测到嗜铬粒蛋白mRNA的痕迹。
