Pagani A, Fischer-Colbrie R, Sanfilippo B, Winkler H, Cerrato M, Bussolati G
Department of Biomedical Sciences and Human Oncology, University of Turin, Italy.
Diagn Mol Pathol. 1992 Sep;1(3):165-72.
Production of chromogranins, the acidic components of the chromaffin granules regarded as specific neuroendocrine markers, was analyzed by immunocytochemistry and hybridization (Northern blotting and in situ hybridization) in primary lesions and cell lines of Ewing's sarcomas, primitive neuroectodermal tumors (PNETs), and neuroblastomas. Antibodies and probes specific for chromogranin A (CgA), chromogranin B (CgB), and secretogranin II (SgII) were used. Ewing's sarcomas and PNETs, unlike neuroblastomas, were negative for CgA and CgB. Two primary Ewing's sarcomas, one primary PNET (an Askin tumor), and one PNET cell line (TC32) were found to strongly express the SgII gene, as shown by the presence of specific mRNA. This result supports the hypothesis that some Ewing's sarcomas represent a most primitive form of neuroectodermal tumor; in addition, it indicates a diagnostic role of SgII in cases of Ewing's sarcomas and PNETs.
嗜铬粒蛋白是嗜铬颗粒的酸性成分，被视为特定的神经内分泌标志物。通过免疫细胞化学和杂交技术（Northern印迹法和原位杂交），对尤因肉瘤、原始神经外胚层肿瘤（PNET）和神经母细胞瘤的原发灶及细胞系中嗜铬粒蛋白的产生情况进行了分析。使用了针对嗜铬粒蛋白A（CgA）、嗜铬粒蛋白B（CgB）和分泌粒蛋白II（SgII）的抗体及探针。与神经母细胞瘤不同，尤因肉瘤和PNET对CgA和CgB呈阴性。如特定mRNA的存在所示，发现两例原发尤因肉瘤、一例原发PNET（阿斯基肿瘤）和一例PNET细胞系（TC32）强烈表达SgII基因。这一结果支持了某些尤因肉瘤代表神经外胚层肿瘤最原始形式的假说；此外，它表明SgII在尤因肉瘤和PNET病例中具有诊断作用。
