Meyer-Siegler K, Fattor R A, Hudson P B
Department of Urology and Pathology, Bay Pines Veterans Affairs Medical Center, Florida, USA.
Diagn Mol Pathol. 1998 Feb;7(1):44-50. doi: 10.1097/00019606-199802000-00008.
The expression of macrophage migration inhibitory factor (MIF) in prostate tissue was investigated by immunohistochemistry (IHC), enzyme-linked immunosorbent assay (ELISA), and Northern blot analysis using a prostate tissue bank. MIF expression was examined in each of the following established prostate tissue categories: prepubertal, pubertal, adult normal, benign hyperplastic (BPH), focal carcinoma within the prostate, and metastatic prostate cancer. IHC showed that all samples tested were positive for MIF protein, which localized to the glandular epithelial cells with no apparent staining of stroma. The most intense staining was observed in the metastatic prostatic adenocarcinoma and the human prostatic adenocarcinoma cell line LNCaP. Using quantitative ELISA, MIF expression was found to be at least three times higher in metastatic adenocarcinoma than in normal, BPH, or focal carcinoma in the adult prostate. This study is the first to report that prostate glandular epithelial cells express MIF. The exact role of MIF in prostate development and disease progression requires further study.
利用前列腺组织库，通过免疫组织化学（IHC）、酶联免疫吸附测定（ELISA）和Northern印迹分析，研究了巨噬细胞移动抑制因子（MIF）在前列腺组织中的表达情况。在以下已确立的前列腺组织类别中分别检测了MIF的表达：青春期前、青春期、成年正常、良性增生（BPH）、前列腺内局灶性癌以及转移性前列腺癌。免疫组织化学显示，所有检测样本的MIF蛋白均呈阳性，该蛋白定位于腺上皮细胞，基质无明显染色。在转移性前列腺腺癌和人前列腺腺癌细胞系LNCaP中观察到最强的染色。使用定量ELISA发现，转移性腺癌中MIF的表达比成年前列腺中的正常、BPH或局灶性癌至少高3倍。本研究首次报道前列腺腺上皮细胞表达MIF。MIF在前列腺发育和疾病进展中的确切作用需要进一步研究。
