Loguercio C, Clot P, Albano E, Argenzio F, Grella A, De Girolamo V, Delle Cave M, Del Vecchio Bianco C, Nardi G
Second University of Naples, Italy.
Ital J Gastroenterol Hepatol. 1997 Apr;29(2):168-73.
The oxidation of ethanol and acetaldehyde enhances the production of various free radicals involved in membrane lipoperoxidation, and decreases glutathione levels.
We evaluated the effects of acute and chronic ethanol use in vivo, with or without the administration of S-adenosyl-methionine (SAME, 2 g I.v.), and the effects of ethanol and acetaldehyde in vitro, on the erythrocyte levels of malonyldialdehyde and glutathione, and of its principal synthesizing enzymes, gamma-glutamyl-cysteine-synthetase and glutathione-synthetase.
Twelve healthy volunteers (age range 26-44 years, median 32 years) and 20 chronic alcohol abusers without liver disease (age range 26-57 years, median 44 years) were studied. Malonyldialdehyde was evaluated by thiobarbituric acid; glutathione and its enzymes by high performance liquid chromatography using a fluorescent detector.
In the healthy subjects, an acute load of ethanol induced a significant decrease in plasma levels of glutathione, which was inhibited by the infusion of S-adenosyl-methionine. In the erythrocytes of alcoholic patients, glutathione and glutathione-synthetase were decreased while malonyldialdehyde was increased. In vitro, acetaldehyde did not affect either the glutathione or the glutathione-related enzyme levels.
Our data suggest that the alterations in glutathione metabolism in the erythrocytes of alcoholics may be due principally to the production of free radicals, as supported by the high levels of malonyldialdehyde observed.
乙醇和乙醛的氧化会增强参与膜脂质过氧化的各种自由基的产生，并降低谷胱甘肽水平。
我们评估了体内急性和慢性乙醇使用（无论是否给予S-腺苷甲硫氨酸（SAME，2 g静脉注射））的影响，以及体外乙醇和乙醛对红细胞中丙二醛、谷胱甘肽及其主要合成酶γ-谷氨酰半胱氨酸合成酶和谷胱甘肽合成酶水平的影响。
研究了12名健康志愿者（年龄范围26 - 44岁，中位数32岁）和20名无肝病的慢性酗酒者（年龄范围26 - 57岁，中位数44岁）。通过硫代巴比妥酸评估丙二醛；通过使用荧光检测器的高效液相色谱法评估谷胱甘肽及其酶。
在健康受试者中，急性乙醇负荷导致血浆谷胱甘肽水平显著降低，而S-腺苷甲硫氨酸的输注可抑制这种降低。在酒精性患者的红细胞中，谷胱甘肽和谷胱甘肽合成酶降低，而丙二醛增加。在体外，乙醛对谷胱甘肽或与谷胱甘肽相关的酶水平均无影响。
我们的数据表明，酗酒者红细胞中谷胱甘肽代谢的改变可能主要归因于自由基的产生，观察到的高水平丙二醛支持了这一点。
