Colantoni A, De Maria N, Idilman R, Van Thiel D H
Department of Gastroenterology and Nutrition, Chandler Medical Center, University of Kentucky, Lexington 40536-0084, USA.
Ital J Gastroenterol Hepatol. 1997 Jun;29(3):273-4.
Polymerase chain reaction is widely used in clinical practice as a reliable assay for the detection and quantitation of hepatitis C virus RNA in serum and tissue. Due to the high sensitivity of the test, both false positive and false negative results can occur. The presence of cryoglobulins in serum samples represents a variable that can influence the result of a polymerase chain reaction assay leading to false negative results. In cases of hepatitis C virus infection associated with cryoglobulinaemia, it is extremely important to very carefully process the samples to be tested in order to avoid cryoglobulin precipitation and the consequent removal of the virus from the sample.
聚合酶链反应作为一种可靠的检测方法，广泛应用于临床实践，用于检测和定量血清及组织中的丙型肝炎病毒RNA。由于该检测的高灵敏度，可能会出现假阳性和假阴性结果。血清样本中冷球蛋白的存在是一个可能影响聚合酶链反应检测结果并导致假阴性结果的变量。在与冷球蛋白血症相关的丙型肝炎病毒感染病例中，极其重要的是要非常小心地处理待检测样本，以避免冷球蛋白沉淀以及随之而来的病毒从样本中去除。
