Caloca M J, Suarez S, Soler J
Departamento de Bioquimica y Biologia Molecular, Universidad de Leon, Spain.
Vet Microbiol. 1998 Mar 15;61(1-2):51-8. doi: 10.1016/s0378-1135(98)00164-3.
The characteristics of the binding of biotinylated E. coli K88ab fimbriae to guinea pig erythrocyte membranes, as a possible model of the target host cell were studied. Binding showed sigmoidal dependence, with an apparent saturation at about 0.8 ng of fimbriae. Hill coefficient values (h) were about 2-2.4, which indicated that the receptor population showed positive cooperativity with at least three binding sites. Apparent binding constants to the first and third binding sites (1K3 and 3K3) were determined. Three K88ab binding proteins, of 67, 63 and 48 kDa, were identified on solubilized erythrocyte membranes and were recovered mainly in a detergent phase, suggesting a possible integral localization of the receptors.
研究了生物素化的大肠杆菌K88ab菌毛与豚鼠红细胞膜的结合特性，以此作为靶宿主细胞的一种可能模型。结合呈现S形依赖性，菌毛约0.8 ng时出现明显饱和。希尔系数值（h）约为2 - 2.4，这表明受体群体具有至少三个结合位点的正协同性。测定了对第一和第三结合位点的表观结合常数（1K3和3K3）。在溶解的红细胞膜上鉴定出三种分子量分别为67 kDa、63 kDa和48 kDa的K88ab结合蛋白，且主要在去污剂相中回收，这表明受体可能定位于膜内。
