Elliott S J, Nandapalan N, Chang B J
Department of Microbiology, University of Western Australia, Nedlands.
Microb Pathog. 1991 Jun;10(6):481-6. doi: 10.1016/0882-4010(91)90114-p.
Escherichia coli HB101 is frequently used as a host in the cloning of bacterial virulence genes because of its reported lack of virulence determinants such as fimbriae, adhesins and haemagglutinins. However, passage of HB101 in standing broth culture rapidly induced the production of fimbriae which mediated adhesion to HEp-2 cells and mannose-sensitive haemagglutination of human and guinea-pig erythrocytes. Fimbrial serology, morphology and pilin molecular mass of 18 kDa were consistent with those of type 1 fimbriae.
由于据报道大肠杆菌HB101缺乏诸如菌毛、黏附素和血凝素等毒力决定因素，它常被用作克隆细菌毒力基因的宿主。然而，将HB101在静置肉汤培养物中传代可迅速诱导菌毛的产生，这些菌毛介导了对HEp-2细胞的黏附以及对人和豚鼠红细胞的甘露糖敏感血凝反应。菌毛血清学、形态学以及18 kDa的菌毛蛋白分子量与1型菌毛相符。
