Keles I, Woldehiwet Z, Murray R D
University of Liverpool, Department of Veterinary Clinical Science and Animal Husbandry, Veterinary Teaching Hospital, Leahurst, Neston, S. Wirral, UK.
Vet Microbiol. 1998 Apr 15;61(4):237-48. doi: 10.1016/s0378-1135(98)00184-9.
The present study compared the replication of bovine respiratory syncytial virus (BRSV) in bovine and ovine peripheral blood mononuclear cells, ovine and bovine monocytic cell lines and ovine alveolar macrophages. Low titres of virus were detected in ovine and bovine lymphocytes and monocytes 24-96 h post-exposure to the virus but there was no apparent replication of the virus in ovine alveolar macrophages during the culture period. The virus replicated to higher but statistically insignificant titres in ovine and bovine peripheral blood monocytes than in lymphocytes, with lymphocytes yielding peak titres significantly earlier. The secondary cell lines obtained from ovine liver and bone marrow also supported the replication of BRSV to high titres. The titres of BRSV in ovine and bovine lymphocytes and monocytes were significantly lower than in secondary cell lines. The addition of human recombinant tumour necrosis factor alpha after exposure to the virus or pre-incubation of ovine or bovine monocytic cells with either human recombinant interleukin 2 or phorbol myristate acetate before exposure to BRSV, did not significantly affect virus titre. Pre-incubation of cells with indomethacin or actinomycin significantly lowered virus titre (p < 0.05).
本研究比较了牛呼吸道合胞病毒（BRSV）在牛和羊外周血单个核细胞、羊和牛单核细胞系以及羊肺泡巨噬细胞中的复制情况。在暴露于病毒后24 - 96小时，在羊和牛的淋巴细胞及单核细胞中检测到低滴度病毒，但在培养期间羊肺泡巨噬细胞中未观察到病毒的明显复制。BRSV在羊和牛外周血单核细胞中的复制滴度高于淋巴细胞，但差异无统计学意义，且淋巴细胞产生峰值滴度的时间明显更早。从羊肝脏和骨髓获得的二代细胞系也支持BRSV的高滴度复制。BRSV在羊和牛淋巴细胞及单核细胞中的滴度显著低于二代细胞系。在暴露于病毒后添加人重组肿瘤坏死因子α，或在暴露于BRSV之前用重组人白细胞介素2或佛波醇肉豆蔻酸酯对羊或牛单核细胞进行预孵育，均未显著影响病毒滴度。用吲哚美辛或放线菌素对细胞进行预孵育可显著降低病毒滴度（p < 0.05）。
