Sharma A K, Woldehiwet Z
Department of Veterinary Pathology, University of Liverpool, Wirral, UK.
Vet Microbiol. 1996 Jan;48(1-2):125-34. doi: 10.1016/0378-1135(95)00131-x.
Adherent and non-adherent mononuclear cells obtained from the peripheral blood of normal lambs supported the replication of bovine respiratory syncytial virus in vitro. Sequential treatment of monocytes with phorbol ester acetate (PMA) enhanced their ability to support viral replication. After exposure in vitro for 24 h, viral antigens were present in 47 +/- 4.5% of monocytes and 32 +/- 3% of lymphocytes. Treatment of monocytes with PMA resulted in the increase of the proportion of cells expressing viral antigen and in the titre of infectious virus.
从正常羔羊外周血中获得的贴壁和非贴壁单核细胞在体外支持牛呼吸道合胞病毒的复制。用佛波酯醋酸盐（PMA）对单核细胞进行连续处理可增强其支持病毒复制的能力。体外暴露24小时后，47±4.5%的单核细胞和32±3%的淋巴细胞中存在病毒抗原。用PMA处理单核细胞导致表达病毒抗原的细胞比例增加以及感染性病毒滴度升高。
