药品中头孢氨苄和头孢克洛的高效薄层色谱法测定

HPTLC assay of cephalexin and cefaclor in pharmaceuticals.

作者信息

Agbaba D, Eric S, Zivanov Stakic D, Vladimirov S

机构信息

Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Belgrade, Serbia, Yugoslavia.

出版信息

Biomed Chromatogr. 1998 May-Jun;12(3):133-5. doi: 10.1002/(SICI)1099-0801(199805/06)12:3<133::AID-BMC780>3.0.CO;2-8.

DOI:10.1002/(SICI)1099-0801(199805/06)12:3<133::AID-BMC780>3.0.CO;2-8

PMID:9646911

Abstract

A simple and reliable HPTLC method for the simultaneous determination of cephalexin and cefaclor is developed and validated. The methanol-ethyl acetate-acetone-water (5:2.5:2.5:1.5 v/v/v/v) solvent system is used for the quantitative evaluation of chromatograms. The chromatographic zones, corresponding to the spots of cephalexin and cefaclor on the silica gel plates, are scanned in the reflectance/absorbance mode at 265 nm. The method is found to be reproducible and convenient for the quantitative analysis of cephalexin and cefaclor in its dosage forms.

摘要

开发并验证了一种同时测定头孢氨苄和头孢克洛的简单可靠的高效薄层色谱法。采用甲醇 - 乙酸乙酯 - 丙酮 - 水（5:2.5:2.5:1.5 v/v/v/v）溶剂系统对色谱图进行定量评估。硅胶板上对应头孢氨苄和头孢克洛斑点的色谱区带在265 nm处以反射率/吸光度模式进行扫描。该方法对于其剂型中头孢氨苄和头孢克洛的定量分析具有可重复性且方便实用。