Qayumi A K, English J C, Godin D V, Ansley D M, Loucks E B, Lee J U, Kim C W
Department of Surgery, The University of British Columbia, Vancouver Hospital and Health Sciences Centre, Canada.
Ann Thorac Surg. 1998 Jun;65(6):1690-7. doi: 10.1016/s0003-4975(98)00275-6.
This swine model was designed to elucidate the role of platelet-activating factor in regional myocardial ischemia-reperfusion injury.
In groups 1 and 2 (n = 12 each), the left anterior descending coronary artery was ligated for 60 minutes to induce regional myocardial ischemia followed by 6 hours of reperfusion. Group 1 received the platelet-activating factor antagonist TCV-309 before ischemia, whereas group 2 did not. Group 3 (n = 3) had a sham operation.
Animals in group 2 exhibited significant (p < 0.05) hemodynamic instability and myocardial depression during the reperfusion period. Despite preventive measures, 7 of the 12 animals experienced severe dysrhythmias in the form of atrial and ventricular fibrillation leading to cardiac arrest. In contrast, animals in group 1 in whom the effects of platelet-activating factor were blocked by the specific platelet-activating factor receptor antagonist TCV-309 were hemodynamically stable and had significantly (p < 0.05) better myocardial function. This significant difference in global myocardial function between the groups was observed in the presence of similar morphologic findings and regional myocardial function.
These results suggest that platelet-activating factor has a definite influence on global myocardial dysfunction associated with regional myocardial ischemia-reperfusion injury.
本猪模型旨在阐明血小板活化因子在局部心肌缺血-再灌注损伤中的作用。
在第1组和第2组（每组n = 12）中，结扎左前降支冠状动脉60分钟以诱导局部心肌缺血，随后再灌注6小时。第1组在缺血前接受血小板活化因子拮抗剂TCV - 309，而第2组未接受。第3组（n = 3）进行假手术。
第2组动物在再灌注期间表现出显著（p < 0.05）的血流动力学不稳定和心肌抑制。尽管采取了预防措施，12只动物中有7只出现了以心房和心室颤动形式的严重心律失常，导致心脏骤停。相比之下，第1组动物中血小板活化因子的作用被特异性血小板活化因子受体拮抗剂TCV - 309阻断，其血流动力学稳定，心肌功能明显（p < 0.05）更好。在存在相似形态学表现和局部心肌功能的情况下，观察到两组之间整体心肌功能存在显著差异。
这些结果表明血小板活化因子对与局部心肌缺血-再灌注损伤相关的整体心肌功能障碍有明确影响。
