Weissgarten J, Modai D, Averbukh M, Cohn M, Berman S, Averbukh Z
Nephrology Division, Assaf Harofeh Medical Center, Zerifin, Israel.
Nephron. 1998;79(2):201-5. doi: 10.1159/000045025.
A high-protein diet is one of the maneuvers which produce hypertrophy of kidney mass. The underlying mechanism(s) has not been elucidated. In the present study, we investigated the possibility that a humoral factor may be involved.
Twenty-eight 3-week-old Charles River rats were studied. Fourteen underwent right nephrectomy and 14 sham operation. Each of these groups was divided into two equal subgroups (n = 7 in each): one maintained on a regular diet (20% protein) and the other on a high-protein diet (60% protein) for 7 days. Following this period the animals were sacrificed. Sera from the animals were added to mesangial cell cultures from kidneys of intact 3-week-old rats, and the thymidine incorporation was assessed.
The parameters of kidney mass indicated that the high-protein diet indeed produced kidney hypertrophy. Sera from the sham-nephrectomized animals fed a high-protein diet produced a significantly greater proliferative effect on mesangial cells in culture than sera from the respective animals on a normal-protein diet. Sera from either nephrectomized group or from the high-protein sham-operated group all had similar magnitudes of enhancement of mesangial cell proliferation.
We conclude that the renal hypertrophy produced by a high-protein diet is mediated, at least in part, by a humoral factor(s).
高蛋白饮食是导致肾脏质量肥大的方法之一。其潜在机制尚未阐明。在本研究中，我们调查了体液因子可能参与其中的可能性。
对28只3周龄的查尔斯河大鼠进行研究。14只大鼠接受右肾切除术，14只进行假手术。这些组中的每一组又分为两个相等的亚组（每组n = 7）：一组维持常规饮食（20%蛋白质），另一组维持高蛋白饮食（60%蛋白质）7天。在此期间过后，处死动物。将动物的血清添加到来自完整3周龄大鼠肾脏的系膜细胞培养物中，并评估胸苷掺入情况。
肾脏质量参数表明高蛋白饮食确实导致肾脏肥大。喂食高蛋白饮食的假肾切除动物的血清对培养中的系膜细胞产生的增殖作用明显大于相应的喂食正常蛋白质饮食动物的血清。肾切除组或高蛋白假手术组的血清对系膜细胞增殖的增强程度相似。
我们得出结论，高蛋白饮食引起的肾脏肥大至少部分是由体液因子介导的。
