Weissgarten J, Modai D, Cohn M, Berman S, Averbukh Z
Nephrology Division, Assaf Harofeh Medical Center Affiliated to Sackler Medical School of Tel Aviv University, Zerifin, Israel.
Nephron. 1998;78(2):212-4. doi: 10.1159/000044913.
Postnephrectomy serum has been proved to enhance proliferation of mesangial cells in culture. Studies in offspring to uninephrectomized (UNx) mothers indicate that it remains active for a long period following UNx. On the other hand, postnephrectomy renal compensatory growth in vivo is known to be completed within a short period. In this study we assessed the proliferative responses of mesangial cells from single kidneys of rats which were cultured 48 h or 4 months following contralateral nephrectomy. The proliferative stimulus was provided by different postnephrectomy sera. Proliferation of both experimental cell populations was significantly greater than that of controls. Moreover, the proliferation rate of early postcontralateral nephrectomy mesangial cells was greater than those harvested 4 months after nephrectomy. We, therefore, propose that the early in vivo completion of single kidney compensatory hypertrophy, at least in part, is due to progressive blunting of renal cell responsiveness to the mitotic stimulus of the animal's serum.
肾切除术后血清已被证明可促进培养的系膜细胞增殖。对单侧肾切除（UNx）母亲的后代进行的研究表明，在UNx后很长一段时间内，该血清仍保持活性。另一方面，已知体内肾切除术后的肾脏代偿性生长在短时间内即可完成。在本研究中，我们评估了对侧肾切除术后48小时或4个月培养的大鼠单肾系膜细胞的增殖反应。增殖刺激由不同的肾切除术后血清提供。两个实验细胞群体的增殖均显著高于对照组。此外，对侧肾切除术后早期系膜细胞的增殖率高于肾切除术后4个月收获的细胞。因此，我们认为单肾代偿性肥大在体内的早期完成，至少部分是由于肾细胞对动物血清有丝分裂刺激的反应性逐渐减弱。
