Jung S I, Han M S, Kwon J H, Cheon C I, Min K H, Lee M S
Department of Biological Science, Sookmyung Women's University, Seoul, Korea.
Biochem Biophys Res Commun. 1998 Jun 29;247(3):741-5. doi: 10.1006/bbrc.1998.8850.
The hisA and hisF genes of Corynebacterium glutamicum were cloned by transforming histidine auxotrophic Escherichia coli with the genomic DNA library. They are two of the eight genes that participate in the histidine biosynthetic pathway. Cloned DNA fragments containing the genes can also complement hisH and hisI auxotrophs of Escherichia coli, suggesting that the four genes are clustered in the genome. We determined the nucleotide sequences of the minimal fragment containing the hisA and hisF genes, which are separated by the impA gene. The coding regions of the hisA and hisF genes are 245 and 257 amino acids in length with a predicted size of about 26 and 27 kDa, respectively. These are in good agreement with the sizes of proteins expressed in E. coli. A high similarity was observed in comparison of nucleotide sequences of each protein between C. glutamicum and other species, as well as those between hisA and hisF genes of C. glutamicum.
通过用谷氨酸棒杆菌的基因组DNA文库转化组氨酸营养缺陷型大肠杆菌，克隆了谷氨酸棒杆菌的hisA和hisF基因。它们是参与组氨酸生物合成途径的八个基因中的两个。含有这些基因的克隆DNA片段也可以互补大肠杆菌的hisH和hisI营养缺陷型，这表明这四个基因在基因组中是成簇的。我们确定了包含hisA和hisF基因的最小片段的核苷酸序列，这两个基因被impA基因隔开。hisA和hisF基因的编码区长度分别为245和257个氨基酸，预测大小分别约为26和27 kDa。这些与在大肠杆菌中表达的蛋白质大小非常一致。在比较谷氨酸棒杆菌与其他物种的每种蛋白质的核苷酸序列以及谷氨酸棒杆菌的hisA和hisF基因之间的核苷酸序列时，观察到高度相似性。
