芽孢杆菌属中编码胸苷酸合成酶A和B的基因属于两个不同的家族。

Genes encoding thymidylate synthases A and B in the genus Bacillus are members of two distinct families.

作者信息

Tam N H, Borriss R

机构信息

Department of Bacterial Genetics, Institute of Biology, Humboldt University, Berlin, Germany.

出版信息

Mol Gen Genet. 1998 May;258(4):427-30. doi: 10.1007/pl00008625.

DOI:10.1007/pl00008625

PMID:9648749

Abstract

Bacillus subtilis strain 168 is known to possess two genes that encode thymidylate synthases, thyA and thyB. We have identified genes similar to the thyA and thyB genes in several Bacillus strains by Southern hybridization and by DNA amplification with sequence-specific primers. Analysis of thyA genes cloned from B. subtilis W23 strain 2A6, B. subtilis ATCC6633, B. amyloliquefaciens S18 and B. atrophaeus S223 reveals that they are very similar to the thyA genes from B. subtilis 168 and its phage phi3T, but differ considerably from the majority of known prokaryotic and eukaryotic thymidylate synthases.

摘要

已知枯草芽孢杆菌168菌株拥有两个编码胸苷酸合成酶的基因，即thyA和thyB。我们通过Southern杂交以及使用序列特异性引物进行DNA扩增，在几种芽孢杆菌菌株中鉴定出了与thyA和thyB基因相似的基因。对从枯草芽孢杆菌W23菌株2A6、枯草芽孢杆菌ATCC6633、解淀粉芽孢杆菌S18和萎缩芽孢杆菌S223克隆的thyA基因进行分析发现，它们与枯草芽孢杆菌168及其噬菌体phi3T的thyA基因非常相似，但与大多数已知的原核和真核胸苷酸合成酶有很大差异。