枯草芽孢杆菌中的两种胸苷酸合成酶。
Two thymidylate synthetases in Bacillus subtilis.
作者信息
Neuhard J, Price A R, Schack L, Thomassen E
出版信息
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1194-8. doi: 10.1073/pnas.75.3.1194.
Abstract
Bacillus subtilis grown at temperatures below 37 degrees contains two thymidylate synthetases, TSaseA and TSaseB. Their presence is dependent on functional thyA and thyB genes, respectively. When cells are grown at 46 degrees they only contain TSaseA activity. This allous an easy positive selection for thyA and thyA, THYB mutants. The two TSases have been physically separated, and they show similar overall requirements for activity. However, they differ significantly in both their kinetic and their physicochemical properties.
摘要
在低于37摄氏度的温度下生长的枯草芽孢杆菌含有两种胸苷酸合成酶，即TSaseA和TSaseB。它们的存在分别依赖于功能性的thyA和thyB基因。当细胞在46摄氏度下生长时，它们只含有TSaseA活性。这便于对thyA和thyA、THYB突变体进行简单的阳性选择。这两种胸苷酸合成酶已被物理分离，并且它们在活性的总体需求上表现相似。然而，它们在动力学和物理化学性质上都有显著差异。
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