Frank G, Zuber H
Hoppe Seylers Z Physiol Chem. 1976 Apr;357(4):585-92. doi: 10.1515/bchm2.1976.357.1.585.
The amino acid sequences of both chains of the sweet protein Monellin have been determined. Since chain separation could not be accomplished easily, cyanogen bromide cleavage at the only methionine residue (in the B-chain) was performed and the three products obtained after cyanogen bromide cleavage were separated. For the identification of amino acid phenylthiohydantoins, high performance liquid chromatography was employed. Thus 37 out of a total of 44 residues of the A chain and 40 out of a total of 42 residues of the large CNBr fragment of the B chain could be determined after Edman degradation of the polypeptides on an automated sequenator.
甜蛋白莫内林两条链的氨基酸序列已被确定。由于链分离不易实现，因此对唯一的甲硫氨酸残基（位于B链）进行了溴化氰裂解，并分离了溴化氰裂解后得到的三种产物。为了鉴定氨基酸苯硫基乙内酰脲，采用了高效液相色谱法。因此，在自动测序仪上对多肽进行埃德曼降解后，可以确定A链总共44个残基中的37个以及B链大的溴化氰片段总共42个残基中的40个。
