Yamamoto T, Katayama I, Nishioka K
Department of Dermatology, Tokyo Medical and Dental University, School of Medicine, 1-5-45 Yushima, Bunkyoku, Tokyo 113-0034, Japan.
Eur J Dermatol. 1998 Jun;8(4):248-51.
Sjögren's syndrome (SjS) is an autoimmune disorder characterized by lymphocytic infiltration into the lacrimal and salivary glands. Annular erythema has recently been reported to be a specific, cutaneous manifestation associated with SjS. In this study, the T cell receptor (TCR) Vbeta gene usage and expansion was examined in annular erythema associated with SjS (AESjS) in 7 patients with primary SjS (5 definite and 2 probable), using reverse transcriptase polymerase chain reaction (RT-PCR) amplification of 22 Vbeta gene families. For 4 out of the 7 patients, the TCR V repertoire in lesional skin of AESjS was compared with paired peripheral blood mononuclear cells (PBL). In one case, two lesional tissue specimens biopsied from different sites of AESjS (face and trunk) were examined. As a control, the TCR Vbeta repertoire was examined from the lesional skin of butterfly rash biopsied from 3 cases of systemic lupus erythematosus (SLE). Results showed that TCR Vbeta 2 was detected in 6 out of the 7 cases of AESjS, although diverse usage was observed. TCR Vbeta 2 and 17 (but particularly Vbeta 2) were predominantly expressed in AESjS in comparison with paired PBL. In the case which presented AESjS at two separate sites, Vbeta 2, 6, 18 and 19 were preferentially expressed in both skin sites as compared with PBL. On the other hand, TCR Vbeta 6, 13-2 and 14 were commonly demonstrated in the cutaneous lesions of SLE. These results suggest that (1) the TCR Vbeta usage by infiltrating T cells in AESjS is not strictly limited, however, Vbeta 2 may play an important role in the induction of AESjS, and that (2) different subsets of TCR Vbeta genes are used in the lesional skin of SjS and SLE, which might account for the clinical and histological differences seen in the erythema found in these two autoimmune disorders.
干燥综合征（SjS）是一种自身免疫性疾病，其特征是淋巴细胞浸润泪腺和唾液腺。环形红斑最近被报道为与SjS相关的一种特异性皮肤表现。在本研究中，使用逆转录聚合酶链反应（RT-PCR）扩增22个Vβ基因家族，对7例原发性干燥综合征（5例确诊和2例可能）患者的与干燥综合征相关的环形红斑（AESjS）中的T细胞受体（TCR）Vβ基因使用情况和扩增进行了检测。对于7例患者中的4例，将AESjS病变皮肤中的TCR V库与配对的外周血单个核细胞（PBL）进行了比较。在1例患者中，检查了从AESjS不同部位（面部和躯干）活检的两个病变组织标本。作为对照，从3例系统性红斑狼疮（SLE）患者活检的蝶形红斑病变皮肤中检测了TCR Vβ库。结果显示，7例AESjS患者中有6例检测到TCR Vβ2，尽管观察到使用情况多样。与配对的PBL相比，TCR Vβ2和17（尤其是Vβ2）在AESjS中占主导地位。在两个不同部位出现AESjS的病例中，与PBL相比，Vβ2、6、18和19在两个皮肤部位均优先表达。另一方面，TCR Vβ6、13-2和14在SLE的皮肤病变中普遍存在。这些结果表明：（1）AESjS中浸润T细胞的TCR Vβ使用情况并非严格受限，然而，Vβ2可能在AESjS的诱导中起重要作用；（2）SjS和SLE病变皮肤中使用了不同的TCR Vβ基因亚群，这可能解释了这两种自身免疫性疾病中红斑的临床和组织学差异。
