人类白细胞测试系统。IX. 用伊格尔最低限度基本培养基（MEM）建立的PHA刺激的2天培养物中的DNA合成和有丝分裂。
The human leukocyte test system. IX. DNA synthesis and mitoses in PHA-stimulated 2-day cultures set up with Eagle's mimimal essential medium (MEM).
作者信息
Obe G, Brandt K, Beek B
出版信息
Hum Genet. 1976 Aug 30;33(3):263-8. doi: 10.1007/BF00286850.
In human leukocyte cultures set up with Eagle's MEM and stimulated with Difco's PHA M, DNA synthesis and mitotic indices were analyzed by means of 3(H)-thymidine autoradiography and cell counting from 23 up to 52 h after culture initiation. Considerable amounts of DNA synthesis and mitoses were found in this time span. This resembles the results found with Ham's F-10 medium. However, the DNA synthesis pattern and the distribution of mitotic indices a higher yield of asynchrony in Eagle's MEM as compared with Ham's F-10 cultures. Proportions of first, second, and third mitoses at 72 h culture time were determined with different methods.
在用伊格尔氏基本培养基（Eagle's MEM）建立并经迪夫科公司的植物血凝素M（Difco's PHA M）刺激的人白细胞培养物中，在培养开始后23至52小时，通过3(H)-胸腺嘧啶核苷放射自显影术和细胞计数分析DNA合成和有丝分裂指数。在此时间段内发现了大量的DNA合成和有丝分裂。这与在哈姆氏F-10培养基（Ham's F-10 medium）中发现的结果相似。然而，与哈姆氏F-10培养物相比，伊格尔氏基本培养基中的DNA合成模式和有丝分裂指数分布具有更高的异步率。用不同方法测定了培养72小时时第一次、第二次和第三次有丝分裂的比例。
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