Crawford J J, Sims G K, Mulvaney R L, Radosevich M
Department of Natural Resources and Environmental Sciences, University of Illinois, Urbana 61801, USA.
Appl Microbiol Biotechnol. 1998 May;49(5):618-23. doi: 10.1007/s002530051223.
Anaerobic biodegradation of atrazine by the bacterial isolate M91-3 was characterized with respect to mineralization, metabolite formation, and denitrification. The ability of the isolate to enhance atrazine biodegradation in anaerobic sediment slurries was also investigated. The organism utilized atrazine as its sole source of carbon and nitrogen under anoxic conditions in fixed-film (glass beads) batch column systems. Results of HPLC and TLC radiochromatography suggest that anaerobic biotransformation of atrazine by microbial isolate M91-3 involved hydroxyatrazine formation. Ring cleavage was demonstrated by 14CO2 evolution. Denitrification was confirmed by detection of 15N2 in headspace samples of K15NO3-amended anaerobic liquid cultures. In aquatic sediments, mineralization of uniformly ring-labeled [14C]atrazine occurred in both M91-3-inoculated and uninoculated sediment. Inoculation of sediments with M91-3 did not significantly enhance anaerobic mineralization of atrazine as compared to uninoculated sediment, which suggests the presence of indigenous organisms capable of anaerobic atrazine biodegradation. Results of this study suggest that the use of M91-3 in a fixed-film bioreactor may have applications in the anaerobic removal of atrazine and nitrate from aqueous media.
对细菌分离株M91 - 3厌氧生物降解阿特拉津的特性进行了研究，涉及矿化、代谢产物形成和反硝化作用。还研究了该分离株在厌氧沉积物浆液中增强阿特拉津生物降解的能力。在固定膜（玻璃珠）间歇柱系统的缺氧条件下，该微生物将阿特拉津用作其唯一的碳源和氮源。高效液相色谱（HPLC）和薄层色谱放射性色谱分析结果表明，微生物分离株M91 - 3对阿特拉津的厌氧生物转化涉及羟基阿特拉津的形成。通过14CO2释放证明了环裂解。通过检测添加K15NO3的厌氧液体培养物顶空样品中的15N2证实了反硝化作用。在水生沉积物中，接种M91 - 3和未接种的沉积物中均发生了均匀环标记的[14C]阿特拉津的矿化。与未接种的沉积物相比，用M91 - 3接种沉积物并没有显著增强阿特拉津的厌氧矿化作用，这表明存在能够厌氧生物降解阿特拉津的本土生物。本研究结果表明，在固定膜生物反应器中使用M91 - 3可能在从水介质中厌氧去除阿特拉津和硝酸盐方面具有应用价值。
