Shen Y, Ford-Lloyd B V, Newbury H J
School of Biological Sciences, University of Birmingham, Edgbaston, UK.
Heredity (Edinb). 1998 May;80 ( Pt 5):624-32. doi: 10.1046/j.1365-2540.1998.00332.x.
The sequences of ITS1 of the internal transcribed spacer regions of nuclear ribosomal DNA from 11 species or subspecies in four sections of the genus Beta were compared. Phylogeny of these wild beet taxa was inferred from the sequence data using phenetic and phylogenetic analyses. Multiple accessions from the same 11 taxa were subjected to random amplified polymorphic DNA (RAPD) analysis, and the data were analysed phenetically. With both molecular techniques and each analysis, three distinctive groups were formed: species from section Beta formed one group; species from section Procumbentes formed a very distinct group; and species from both section Nanae and section Corollinae clustered together forming the third group, which is closer to Beta than Procumbentes. The RAPD data revealed within-section interspecies relationships that are consistent with those reported previously; this was not always the case using the single-locus sequence data.
比较了甜菜属四个组中11个种或亚种的核糖体DNA内转录间隔区ITS1的序列。利用表型分析和系统发育分析，从序列数据推断这些野生甜菜类群的系统发育。对来自相同11个类群的多个样本进行随机扩增多态性DNA（RAPD）分析，并对数据进行表型分析。通过这两种分子技术和每种分析，形成了三个不同的组：甜菜组的物种形成一组；匍匐组的物种形成一个非常独特的组；矮生组和花冠组的物种聚集在一起形成第三组，该组比匍匐组更接近甜菜组拉。RAPD数据揭示的组内种间关系与先前报道的一致；而使用单基因座序列数据时情况并非总是如此。
