Tagusari O, Yamazaki K, Litwak P, Kojima A, Klein E C, Antaki J F, Watach M, Gordon L M, Kono K, Mori T, Koyanagi H, Griffith B P, Kormos R L
Department of Surgery, University of Pittsburgh Medical Center, Pennsylvania 15219, USA.
Artif Organs. 1998 Jun;22(6):481-7. doi: 10.1046/j.1525-1594.1998.06152.x.
The development of a percutaneous artificial internal organ system requires a reliable biocompatible connection between the external environment and the inside of the human body. Such is necessary for the success of a permanent left ventricular assist device. However, the search for a satisfactory interface at the epidermal level has proven to be difficult. Carbon has been proposed for this application, but its texture does not typically promote ingrowth from surrounding tissue. We have therefore employed a new processing method to produce a fine trabecularized carbon implant. The method for preparing the implant involves infiltrating low temperature pyrolytic carbon into the surface of a carbon core which is wrapped with carbon fabric. This results in a tightly woven porous structure of carbon (carbon fiber diameter: 35-50 microm, maximal pore size >200 microm) with gradually increasing porosity from 15-75%. We implanted test samples percutaneously in a calf for in vivo histological evaluation. Thirty days after implantation epidermal downgrowth was minimal. Microscopic analysis revealed that a thin fibrous capsule surrounded the implant, and mature connective tissue with accompanying blood vessels filled the pores of the fine trabecularized carbon layer. From these results we suggest that fine trabecularized carbon is ideally suited for a percutaneous device system in a permanent left ventricular assist device.
经皮人工内脏系统的研发需要在外部环境与人体内部之间建立可靠的生物相容性连接。这对于永久性左心室辅助装置的成功至关重要。然而，事实证明在表皮层面寻找令人满意的界面颇具难度。有人提议将碳用于此应用，但它的质地通常不利于周围组织向内生长。因此，我们采用了一种新的加工方法来制造精细的小梁化碳植入物。制备该植入物的方法包括将低温热解碳渗入包裹有碳纤维织物的碳芯表面。这会形成一种紧密编织的多孔碳结构（碳纤维直径：35 - 50微米，最大孔径>200微米），孔隙率从15%逐渐增加到75%。我们将测试样品经皮植入小牛体内进行组织学评估。植入30天后，表皮向内生长极少。显微镜分析显示，植入物周围有一层薄纤维囊，成熟的结缔组织及伴行血管填充了精细小梁化碳层的孔隙。基于这些结果，我们认为精细小梁化碳非常适合用于永久性左心室辅助装置中的经皮装置系统。
