Ashton T, Rowlands C C, Jones E, Young I S, Jackson S K, Davies B, Peters J R
Department of Health and Exercise Science, School of Applied Sciences, University of Glamorgan, Pontypridd, Wales, UK.
Eur J Appl Physiol Occup Physiol. 1998 May;77(6):498-502. doi: 10.1007/s004210050366.
Free radicals or oxidants are continuously produced in the body as a consequence of normal energy metabolism. The concentration of free radicals, together with lipid peroxidation, increases in some tissues as a physiological response to exercise - they have also been implicated in a variety of pathologies. The biochemical measurement of free radicals has relied in the main on the indirect assay of oxidative stress by-products. This study presents the first use of electron spin resonance (ESR) spectroscopy in conjunction with the spin-trapping technique, to measure directly the production of radical species in the venous blood of healthy human volunteers pre- and post-exhaustive aerobic exercise. Evidence is also presented of increased lipid peroxidation and total antioxidant capacity post-exercise.
作为正常能量代谢的结果，自由基或氧化剂在体内持续产生。作为对运动的生理反应，某些组织中自由基的浓度以及脂质过氧化作用会增加——它们也与多种病理状况有关。自由基的生化测量主要依赖于对氧化应激副产物的间接测定。本研究首次使用电子自旋共振（ESR）光谱结合自旋捕获技术，直接测量健康人类志愿者在进行力竭性有氧运动前后静脉血中自由基的产生情况。同时也有证据表明运动后脂质过氧化和总抗氧化能力增加。
