Bruckner I, Serban M, Matei I, Nichifor E, Vlădescu C, Mitu S, Cucu C
Med Interne. 1978 Apr-Jun;16(2):117-25.
Comparative studies aiming at the detection of certain tubular protein elements by means of Ouchterlony's immunodiffusion, in parallel with lysozyme and guanase assays, were carried out in the unconcentrated urines of 746 subjects, of whom 655 apparently healthy inhabitants (mostly children) from a region with endemic nephropathy (EN) and from Bucharest, as well as 91 adults with EN or various other diseases with renal involvement. The presence of light chains, of lysozymuria exceeding 2 microgram/ml, of beta2 microglobulin and of guanase in the urines of children and adults from the endemic area was significantly more frequent than in the control groups. These immunochemical changes are hence considered as valuable criteria for the detection of EN prior to the uremic stage. They should be looked for, first and foremost, in the young relatives of patients with this disease. In the stage of nitrogen retention the diagnostic value of these tests is reduced, since the same changes can also be found in the urines of patients suffering from other diseases with renal involvement, which show nitrogen retention as well.
通过欧氏免疫扩散法并结合溶菌酶和鸟嘌呤酶检测，对746名受试者的未浓缩尿液进行了比较研究，以检测某些肾小管蛋白成分。其中包括655名来自地方性肾病（EN）流行地区和布加勒斯特的表面健康居民（大多为儿童），以及91名患有EN或其他各种累及肾脏疾病的成年人。来自流行地区的儿童和成人尿液中轻链、溶菌酶尿超过2微克/毫升、β2微球蛋白和鸟嘌呤酶的出现频率明显高于对照组。因此，这些免疫化学变化被认为是在尿毒症阶段之前检测EN的有价值标准。首先应在患有这种疾病患者的年轻亲属中寻找这些变化。在氮潴留阶段，这些检测的诊断价值会降低，因为在患有其他累及肾脏且也有氮潴留的疾病患者的尿液中也能发现同样的变化。
