Müller S C, Mair T, Steinbock O
Otto-von-Guericke-Universität, Abteilung Biophysik, Magdeburg, Germany.
Biophys Chem. 1998 May 5;72(1-2):37-47. doi: 10.1016/s0301-4622(98)00121-5.
Biological self-organization was investigated in a biochemical and a cellular system: yeast extract and cultures of the slime mold Dictyostelium discoideum. In both systems traveling reaction-diffusion waves occur in response to oscillatory reactions. Glycolytic degradation of sugar in a yeast extract leads to the spontaneous formation of NADH and proton waves. Manipulation of the adenine nucleotide pool by addition of purified plasma membrane ATPase favors the formation of both reaction-diffusion waves and phase waves. The results indicate that the energy charge has an important impact for the dynamics of glycolytic patterns. When affecting the lower part of glycolysis by pyruvate addition the frequency of wave generation was increased with concomitant formation of rotating NADH and proton spirals. During morphogenesis of the cellular system Dictyostelium discoideum, circular and spiral shaped aggregation patterns of motile amoeboid cells form in response to traveling cAMP waves. Velocity analysis of the cell movements reveals that the cAMP waves guide the cells towards the site of wave initiation along optimized trajectories. The minimization of aggregation paths is based on a mechanism exploiting general properties of excitation waves. The resulting aggregation territories are reminiscent of Voronoi diagrams.
酵母提取物和黏菌盘基网柄菌的培养物。在这两个系统中，响应振荡反应会出现行波反应扩散波。酵母提取物中糖的糖酵解降解会导致NADH和质子波的自发形成。通过添加纯化的质膜ATP酶来操纵腺嘌呤核苷酸库有利于反应扩散波和相位波的形成。结果表明，能荷对糖酵解模式的动力学有重要影响。当通过添加丙酮酸影响糖酵解的下游部分时，波产生的频率增加，同时形成旋转的NADH和质子螺旋。在细胞系统盘基网柄菌的形态发生过程中，响应行波cAMP波，运动性变形虫样细胞形成圆形和螺旋形聚集模式。对细胞运动的速度分析表明，cAMP波沿着优化的轨迹将细胞导向波起始位点。聚集路径的最小化基于一种利用激发波一般特性的机制。由此产生的聚集区域让人联想到沃罗诺伊图。
