Petty H R, Kindzelskii A L
Department of Biological Sciences, Wayne State University, Detroit, MI 48202, USA.
Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3145-9. doi: 10.1073/pnas.061014298. Epub 2001 Mar 6.
Self-organization is a common theme in biology. One mechanism of self-organization is the creation of chemical patterns by the diffusion of chemical reactants and their nonlinear interactions. We have recently observed sustained unidirectional traveling chemical redox [NAD(P)H - NAD(P)(+)] waves within living polarized neutrophils. The present study shows that an intracellular metabolic wave responds to formyl peptide receptor agonists, but not antagonists, by splitting into two waves traveling in opposite directions along a cell's long axis. Similar effects were noted with other neutrophil-activating substances. Moreover, when cells were exposed to an N-formyl-methionyl-leucyl-phenylalanine (FMLP) gradient whose source was perpendicular to the cell's long axis, cell metabolism was locally perturbed with reorientation of the pattern in a direction perpendicular to the initial cellular axis. Thus, extracellular activating signals and the signals' spatial cues are translated into distinct intracellular dissipative structures.
自组织是生物学中的一个常见主题。自组织的一种机制是通过化学反应物的扩散及其非线性相互作用产生化学模式。我们最近在活的极化中性粒细胞内观察到持续的单向传播化学氧化还原[NAD(P)H - NAD(P)(+)]波。本研究表明，细胞内代谢波通过沿细胞长轴分裂为两个相反方向传播的波来响应甲酰肽受体激动剂，而不是拮抗剂。其他中性粒细胞激活物质也有类似作用。此外，当细胞暴露于源垂直于细胞长轴的N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（FMLP）梯度时，细胞代谢会受到局部干扰，模式会在垂直于初始细胞轴的方向上重新定向。因此，细胞外激活信号及其空间线索被转化为不同的细胞内耗散结构。
