Peyrin E, Guillaume Y C, Morin N, Guinchard C
Laboratoire de Chimie Analytique, Faculté de Medecine et Pharmacie, Besancon, France.
J Chromatogr A. 1998 May 29;808(1-2):113-20. doi: 10.1016/s0021-9673(98)00117-4.
The effect of perchlorate anion as a mobile phase modifier on the retention of dansyl norvaline and dansyl tryptophan enantiomers on a human serum albumin (HSA) column was studied by varying the chaotropic agent concentrations. The thermodynamic parameters for the transfer of a solute from the mobile to the HSA stationary phases were determined from linear van't Hoff plots. An enthalpy-entropy compensation study revealed that the type of interaction between the solute and HSA was independent of the molecular structure. The parabolic variations observed with the enthalpic and entropic terms of dansyl amino acid transfer in relation to the concentration of perchlorate anion were considered to be the result of the change from reversed to normal-phase conditions for this chromatographic system.
通过改变离液剂浓度，研究了高氯酸根阴离子作为流动相改性剂对丹磺酰基正缬氨酸和丹磺酰基色氨酸对映体在人血清白蛋白（HSA）柱上保留的影响。从线性范特霍夫图确定了溶质从流动相转移到HSA固定相的热力学参数。焓-熵补偿研究表明，溶质与HSA之间的相互作用类型与分子结构无关。丹磺酰基氨基酸转移的焓项和熵项随高氯酸根阴离子浓度的抛物线变化被认为是该色谱系统从反相条件转变为正相条件的结果。
