André C, Thomassin M, Truong T T, Robert J F, Guillaume Y C
Laboratoire de Chimie Analytique, Faculté de Médecine et de Pharmacie, Place Saint-Jacques, 25030 Besançon cedex, France.
J Chromatogr Sci. 2003 Jul;41(6):311-6. doi: 10.1093/chromsci/41.6.311.
The retention mechanism for a series of D,L-dansyl amino acids in high-performance liquid chromatography is investigated using a teicoplanin stationary phase and hydroxypropyl-beta-cyclodextrin (HP-beta-CD) as the mobile phase additive (0-16mM). A theoretical treatment is developed to determine the HP-beta-CD influence on the equilibrium between the teicoplanin phase and the aqueous medium, respectively. From the experimental data, the association constants of the D,L-dansyl amino acids-HP-beta-CD inclusion complexes are determined and discussed in relation to the enantiomer structure. A thermodynamic study confirms that both the retention and complexation mechanisms are independent of the dansyl amino acid molecular structure and its absolute carbon configuration.
