Jensen-Jarolim E, Santner B, Leitner A, Grimm R, Scheiner O, Ebner C, Breiteneder H
Department of General and Experimental Pathology, University Hospital AKH, Vienna, Austria.
Int Arch Allergy Immunol. 1998 Jun;116(2):103-9. doi: 10.1159/000023932.
Little is known about the role of bell peppers in food allergy. We collected sera from 11 patients with food allergy to bell peppers to analyze bell pepper extracts for allergen composition.
Proteins of mature fruits of eight horticultural strains of bell peppers were extracted and tested with patients' sera for IgE binding and with monoclonal and polyclonal antibodies in immunoblot.
Profilin was detected in bell pepper extracts by an anti-celery profilin antibody. It showed high IgE binding activity in all extracts, which could be inhibited by recombinant birch pollen profilin. Anti-birch pollen monoclonal antibody BIP3, directed against birch pollen proteins between 30 and 69 kD, bound to bell pepper antigens of comparable molecular weights. A homologue of the major birch pollen allergen Bet v 1 was detected in four of eight horticultural strains of bell peppers, and was shown to bind IgE in 1 of the 11 patients. A 23-kD allergen of bell peppers was shown to correspond to the 23-kD major paprika allergen by IgE absorption experiments. Its N-terminal sequence showed 100% identity to P23 from tomatoes.
The appearance of profilin in all and Bet v 1 in 50% of the tested horticultural strains indicates that bell peppers have to be considered potentially dangerous for Bet v 1- and profilin-sensitized patients. Moreover, in 4 of 8 horticultural strains of bell peppers a homologue of the osmotin-like protein P23 from tomatoes is responsible for substantial IgE binding. Contact with Bet v 1 and P23 homologues in bell peppers can therefore be minimized by avoidance of the respective horticultural strains.
关于甜椒在食物过敏中的作用，人们了解甚少。我们收集了11名对甜椒过敏的患者的血清，以分析甜椒提取物中的过敏原成分。
提取了8个园艺品种甜椒成熟果实中的蛋白质，用患者血清检测其IgE结合情况，并在免疫印迹中用单克隆抗体和多克隆抗体进行检测。
用抗芹菜肌动蛋白结合蛋白抗体在甜椒提取物中检测到了肌动蛋白结合蛋白。它在所有提取物中均表现出高IgE结合活性，可被重组桦树花粉肌动蛋白结合蛋白抑制。抗桦树花粉单克隆抗体BIP3针对30至69 kD之间的桦树花粉蛋白，与分子量相当的甜椒抗原结合。在8个园艺品种的甜椒中有4个检测到主要桦树花粉过敏原Bet v 1的同源物，在11名患者中有1名患者的血清显示该同源物能结合IgE。通过IgE吸收实验表明，甜椒的一种23-kD过敏原与主要辣椒粉过敏原23-kD相对应。其N端序列与番茄的P23有100%的同一性。
所有测试园艺品种甜椒中均出现肌动蛋白结合蛋白，50%的品种中出现Bet v 1，这表明对于对Bet v 1和肌动蛋白结合蛋白敏感的患者，甜椒可能具有潜在危险性。此外，在8个园艺品种的甜椒中有4个，番茄中类似渗透素的蛋白P23的同源物是大量IgE结合的原因。因此，通过避免接触相应的园艺品种，可以尽量减少与甜椒中Bet v 1和P23同源物的接触。
