Heiss S, Fischer S, Müller W D, Weber B, Hirschwehr R, Spitzauer S, Kraft D, Valenta R
Institute of General and Experimental Pathology, AKH, University of Vienna, Austria.
J Allergy Clin Immunol. 1996 Nov;98(5 Pt 1):938-47. doi: 10.1016/s0091-6749(96)80010-8.
Cross-reactive IgE antibodies were found to be responsible for allergic reactions in patients allergic to pollen on ingestion of food (oral allergy syndrome). So far, the major birch pollen allergen Bet v 1 and birch profilin (Bet v 2) were identified as relevant cross-reactive allergens.
In this study we attempted to identify additional cross-reactive plant allergens, which could be responsible for food intolerance in patients allergic to pollen.
Monoclonal antibodies specific for the major mugwort pollen allergen, Art v 1, representing a 60 kd glycoprotein, were used to detect cross-reactive allergens in other pollens and plant-derived food. The amino acid compositions of the cross-reactive structures were determined, and their resistance against trypsin treatment was investigated. In addition, IgE immunoblot inhibitions were done with the 60 kd mugwort pollen allergen.
Monoclonal antibodies specific for the major mugwort pollen allergen, Art v 1, cross-reacted with proteins of comparable molecular weight in fruit and vegetables. Preadsorption of patients' sera with the 60 kd mugwort allergen led to a reduction of IgE binding to components of a similar molecular weight present in different pollen (birch, timothy grass), fruit (apple, peanuts), and vegetable (celery) extracts and reduced IgE binding to apple, kiwi, and celery as determined by RAST inhibitions.
A cross-reactive plant panallergen, possibly identical to the major mugwort pollen allergen, Art v 1, is described. The allergen represents a protein of approximately 60 kd present in various pollen and plant foods; which is distinct from Bet v 1 and profilin and hence may represent a novel cross-reactive allergen in the oral allergy syndrome.
已发现交叉反应性IgE抗体是对花粉过敏的患者在摄入食物时发生过敏反应（口腔过敏综合征）的原因。到目前为止，主要的桦树花粉过敏原Bet v 1和桦树肌动蛋白结合蛋白（Bet v 2）被确定为相关的交叉反应性过敏原。
在本研究中，我们试图鉴定出其他可能导致对花粉过敏患者出现食物不耐受的交叉反应性植物过敏原。
使用对主要艾蒿花粉过敏原Art v 1（一种60kd糖蛋白）具有特异性的单克隆抗体，检测其他花粉和植物性食物中的交叉反应性过敏原。测定交叉反应性结构中的氨基酸组成，并研究它们对胰蛋白酶处理的抗性。此外，用60kd艾蒿花粉过敏原进行IgE免疫印迹抑制试验。
对主要艾蒿花粉过敏原Art v 1具有特异性的单克隆抗体与水果和蔬菜中分子量相当的蛋白质发生交叉反应。用60kd艾蒿过敏原预先吸附患者血清，导致IgE与不同花粉（桦树、梯牧草）、水果（苹果、花生）和蔬菜（芹菜）提取物中存在的类似分子量成分的结合减少，并且通过RAST抑制试验测定，IgE与苹果、猕猴桃和芹菜的结合也减少。
描述了一种交叉反应性植物泛过敏原，可能与主要的艾蒿花粉过敏原Art v 1相同。该过敏原是一种存在于各种花粉和植物性食物中的约60kd蛋白质；它与Bet v 1和肌动蛋白结合蛋白不同，因此可能是口腔过敏综合征中一种新的交叉反应性过敏原。
